4 results on '"Engelen-Lee, Joo-Yeon"'
Search Results
2. Microglial Activation After Systemic Stimulation With Lipopolysaccharide and Escherichia coli.
- Author
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Hoogland, Inge C. M., Westhoff, Dunja, Engelen-Lee, Joo-Yeon, Melief, Jeroen, Valls Serón, Mercedes, Houben-Weerts, Judith H. M. P., Huitinga, Inge, van Westerloo, David J., van der Poll, Tom, van Gool, Willem A., and van de Beek, Diederik
- Subjects
MICROGLIA ,LIPOPOLYSACCHARIDES ,ESCHERICHIA coli ,INTRAPERITONEAL injections ,INFLAMMATORY mediators - Abstract
Background: Microglial activation after systemic infection has been suggested to mediate sepsis-associated delirium. A systematic review of animal studies suggested distinct differences between microglial activation after systemic challenge with live bacteria and lipopolysaccharide (LPS). Here, we describe a mouse model of microglial activation after systemic challenge with live Escherichia coli (E. coli) and compare results with systemic challenge with LPS. Methods: Sixty mice were intraperitoneally injected with E. coli (1 × 10
4 colony-forming units) and sacrificed at 12, 20, 48, and 72 h after inoculation. For 48 and 72 h time points, mice were treated with ceftriaxone. Thirty mice were intraperitoneally injected with LPS (5 mg/kg) and sacrificed 3 and 48 h after inoculation; 48 control mice were intraperitoneally injected with isotonic saline. Microglial response wasmonitored by immunohistochemical staining with Iba-1 antibody and flow cytometry; and inflammatory response by mRNA expression of pro- and anti-inflammatory mediators. Results: Mice infected with live E. coli showed microglial activation 72 h post-inoculation, with increased cell number in cortex (p = 0.0002), hippocampus (p = 0.003), and thalamus (p = 0.0001), but not in the caudate nucleus/putamen (p = 0.33), as compared to controls. At 72 h, flow cytometry of microglia from E. coli infected mice showed increased cell size (p = 0.03) and CD45 expression (p = 0.03), but no increase in CD11b expression, and no differences in brain mRNA expression of inflammatory mediators as compared to controls. In mice with systemic LPS stimulation, microglial cells were morphologically activated at the 48 h time point with increased cell numbers in cortex (p = 0.002), hippocampus (p = 0.0003), thalamus (p = 0.007), and caudate nucleus/putamen (p < 0.0001), as compared to controls. At 48 h, flow cytometry of microglia from LPS stimulated mice showed increased cell size (p = 0.03), CD45 (p = 0.03), and CD11b (p = 0.04) expression. Brain mRNA expression of TNF-α (p = 0.02), IL-1β (p = 0.02), and MCP-1 (p = 0.03) were increased as compared to controls. Interpretation: Systemic challenge with live E. coli causes a neuro-inflammatory response, but this response occurs at a later time point and is less vigorous as compared to LPS stimulation. The E. coli model mimics the clinical situation of infection associated delirium more closely than stimulation with supra-natural LPS. [ABSTRACT FROM AUTHOR]- Published
- 2018
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3. Aging and Microglial Response following Systemic Stimulation with Escherichia coli in Mice.
- Author
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Hoogland, Inge C.M., Westhoff, Dunja, Engelen-Lee, Joo-Yeon, Valls Seron, Mercedes, Houben-Weerts, Judith H.M.P., van Westerloo, David J., van der Poll, Tom, van Gool, Willem A., van de Beek, Diederik, and Tozzi, Alessandro
- Subjects
ESCHERICHIA coli ,MICE ,IMMUNOSTAINING ,GENES ,MICROGLIA - Abstract
Systemic infection is an important risk factor for the development cognitive impairment and neurodegeneration in older people. Animal experiments show that systemic challenges with live bacteria cause a neuro-inflammatory response, but the effect of age on this response in these models is unknown. Young (2 months) and middle-aged mice (13–14 months) were intraperitoneally challenged with live Escherichia coli (E. coli) or saline. The mice were sacrificed at 2, 3 and 7 days after inoculation; for all time points, the mice were treated with ceftriaxone (an antimicrobial drug) at 12 and 24 h after inoculation. Microglial response was monitored by immunohistochemical staining with an ionized calcium-binding adaptor molecule 1 (Iba-1) antibody and flow cytometry, and inflammatory response by mRNA expression of pro- and anti-inflammatory mediators. We observed an increased microglial cell number and moderate morphologically activated microglial cells in middle-aged mice, as compared to young mice, after intraperitoneal challenge with live E. coli. Flow cytometry of microglial cells showed higher CD45 and CD11b expressions in middle-aged infected mice compared to young infected mice. The brain expression levels of pro-inflammatory genes were higher in middle-aged than in young infected mice, while middle-aged infected mice had similar expression levels of these genes in the systemic compartment. We conclude that systemic challenge with live bacteria causes an age-dependent neuro-inflammatory and microglial response. Our data show signs of an age-dependent disconnection of the inflammatory transcriptional signature between the brain and the systemic compartment. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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4. Microglial response in triggering receptor expressed on myeloid cells 2 (TREM2) knock-out mice after systemic stimulation with Escherichia coli.
- Author
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Hoogland, Inge C.M., Yik, Jutka, Westhoff, Dunja, Engelen-Lee, Joo-Yeon, Valls Seron, Merche, Man, Wing-Kit, Houben-Weerts, Judith H.P.M., Tanck, Michael W., van Westerloo, David J., van der Poll, Tom, van Gool, Willem A., and van de Beek, Diederik
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KNOCKOUT mice , *MYELOID cells , *ESCHERICHIA coli , *MITOGEN-activated protein kinases , *CEFTRIAXONE , *MICROGLIA , *THALAMIC nuclei - Abstract
• Loss of function of TREM2 during systemic infection leads to an increased number of microglial cells in thalamus after inoculation. • Loss of function of TREM2 during systemic infection does not lead to increased expression of pro-inflammatory genes in the brain. • The role of TREM2 in the neuro-inflammatory response following systemic infection appears to be limited in our experiments. Systemic infection is an important risk factor for delirium, associated with neurodegeneration and subsequent cognitive impairment in older people. Microglial cell response is a known key player in this process and we hypothesize that the triggering receptor expressed on myeloid cells 2 (TREM2) plays an important role in the regulation of this response. 8- to 10-week old male wild-type (WT) and TREM2 knock-out (Trem2 -/-) mice were intraperitoneally inoculated with live Escherichia coli (E. coli) or saline. After inoculation, all mice were treated with ceftriaxone (an antimicrobial drug) at 12 and 24 h and were sacrificed after 2 and 3 days. Microglial response was determined by immunohistochemical staining with an ionized calcium-binding adaptor molecule 1 (Iba-1) antibody and flow cytometry. mRNA expression of pro- and anti-inflammatory mediators was measured to quantify the inflammatory response. We observed increased Iba-1 positive cells number in thalamus of Trem2 -/- mice at 3d after inoculation compared to WT mice (mean 120 cell/mm2 [SD 8] vs 105 cell/mm2 [SD 11]; p = 0.03). Flow cytometry showed no differences in forward scatter or expression of CD11b, CD45 and CD14 between WT and Trem2 -/- mice. The brain mRNA expression levels of tumor necrosis factor alpha (TNF-α) of Trem2 -/- mice at 2d were higher compared to WT mice (p = 0.003). Higher mRNA expression of interleukin 1 beta (IL-1β), Iba-1, CD11b and mitogen-activated protein kinase 1 (MAPK-1) was found in brain of WT mice at 2d compared to Trem2 -/- mice (respectively p = 0.02; p = 0.001; p = 0.03 and p = 0.02). In spleen there were no differences in inflammatory mediators, between WT and Trem2 -/- mice. Although the loss of function of TREM2 during systemic infection led to an increased number of activated microglia in the thalamus, we did not observe a consistent increase in expression of inflammatory genes in the brain. The role of TREM2 in the neuro-inflammatory response following systemic infection therefore appears to be limited. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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