1. Comparison among different preclinical models derived from the same patient with a non-functional pancreatic neuroendocrine tumor.
- Author
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Wang Y, Ye Z, Lou X, Xu J, Jing D, Zhou C, Qin Y, Chen J, Xu X, Yu X, and Ji S
- Subjects
- Humans, Animals, Disease Models, Animal, Mice, Heterografts, Cell Line, Tumor, Organoids, Exome Sequencing, Pancreatic Neoplasms pathology, Pancreatic Neoplasms genetics, Pancreatic Neoplasms therapy, Neuroendocrine Tumors pathology, Neuroendocrine Tumors genetics, Neuroendocrine Tumors therapy
- Abstract
Pancreatic neuroendocrine tumors are the second most common tumors of the pancreas, and approximately half of patients are diagnosed with liver metastases. Currently, the improvement in the efficacy of relevant treatment methods is still limited. Therefore, there is an urgent need for in-depth research on the molecular biological mechanism of pancreatic neuroendocrine tumors. However, due to their relatively inert biology, preclinical models are extremely scarce. Here, the patient-derived organoid, and patient-derived xenograft were successfully constructed. These two models and the previously constructed cell line named SPNE1 all derived from the same patient with a grade 3 non-functional pancreatic neuroendocrine tumor, providing new tumor modeling platforms, and characterized using immunohistochemistry, whole-exome sequencing, and single-cell transcriptome sequencing. Combined with a tumor formation experiment in immunodeficient mice, we selected the model that most closely recapitulated the parental tumor. Overall, the patient-derived xenograft model most closely resembled human tumor tissue., (© 2024. The Author(s) under exclusive licence to Japan Human Cell Society.)
- Published
- 2024
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