1. Effects of Brn-4 on the neuronal differentiation of neural stem cells derived from rat midbrain.
- Author
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Tan XF, Qin JB, Jin GH, Tian ML, Li HM, Zhu HX, Zhang XH, Shi JH, and Huang Z
- Subjects
- Animals, Blotting, Western, Clone Cells, Fluorescent Antibody Technique, Luminescent Proteins metabolism, RNA Interference, Rats, Rats, Sprague-Dawley, Recombinant Proteins metabolism, Stem Cells metabolism, Transfection, Cell Differentiation, Mesencephalon cytology, Nerve Tissue Proteins metabolism, Neurons cytology, Neurons metabolism, POU Domain Factors metabolism, Stem Cells cytology
- Abstract
NSCs (neural stem cells) provide a powerful research tool for the design and discovery of new approaches to cell replacement therapy during brain repair. However, the usefulness of this tool has been particularly obstructed by limited neuronal differentiation of NSCs. Brn-4, a member of the POU domain family of transcription factors, has been previously implicated in the development of neurons by expression analysis. Here, we directly investigated the effects of Brn-4 on the neuronal differentiation and development of NSCs derived from the E13 rat midbrain. We found that Brn-4 knockdown in NSCs resulted in a significant decrease of MAP-2-positive neurons with immature morphology. Overexpression of Brn-4 in NSCs markedly increased the production and maturation of newborn neurons. These results suggest that Brn-4 has a critical role in the neuronal differentiation of mesencephalic NSCs and the maturation of newborn neurons. Brn-4 may be utilized to manipulate NSCs for gene and cell therapy of several neurological diseases.
- Published
- 2010
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