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4 results on '"Katarzyna Sobczuk"'

1. Association of low ficolin-2 concentration in cord serum with respiratory distress syndrome in preterm newborns

Author

Gabriela Gajek, Anna S. Świerzko, Dariusz Jarych, Damian Mikulski, Paulina Kobiela, Karolina Chojnacka, Maja Kufelnicka-Babout, Agnieszka Szala-Poździej, Jędrzej Chrzanowski, Katarzyna Sobczuk, Wojciech Fendler, Misao Matsushita, Iwona Domżalska-Popadiuk, Jan Mazela, Jarosław Kalinka, Hideharu Sekine, and Maciej Cedzyński

Subjects
FCN2, ficolin-2, newborn, prematurity, preterm, respiratory distress syndrome (RDS), Immunologic diseases. Allergy, RC581-607
Abstract

IntroductionFicolin-2 is a serum pattern recognition molecule, involved in complement activation via the lectin pathway. This study aimed to investigate the association of ficolin-2 concentration in cord blood serum with complications related to premature birth.Methods546 premature neonates were included. The concentration of ficolin-2 in cord blood serum was determined by a sandwich TRIFMA method. FCN2 genetic variants were analysed with RFLP-PCR, allele-specific PCR, Sanger sequencing or allelic discrimination using TaqMan probes method.FindingsCord blood serum ficolin-2 concentration correlated positively with Apgar score and inversely with the length of hospitalisation and stay at Neonatal Intensive Care Unit (NICU). Multivariate logistic regression analysis indicated that low ficolin-2 increased the possibility of respiratory distress syndrome (RDS) diagnosis [OR=2.05, 95% CI (1.24-3.37), p=0.005]. Median ficolin-2 concentration was significantly lower in neonates with RDS than in premature babies without this complication, irrespective of FCN2 gene polymorphisms localised to promoter and 3’untranslated regions: for patients born

Published
2023
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2. Polymorphisms of the FCN2 Gene 3’UTR Region and Their Clinical Associations in Preterm Newborns

Author

Anna S. Świerzko, Dariusz Jarych, Gabriela Gajek, Karolina Chojnacka, Paulina Kobiela, Maja Kufelnicka-Babout, Mateusz Michalski, Katarzyna Sobczuk, Agnieszka Szala-Poździej, Misao Matsushita, Jan Mazela, Iwona Domżalska-Popadiuk, David C. Kilpatrick, Jarosław Kalinka, Hideharu Sekine, and Maciej Cedzyński

Subjects
3’UTR, ficolin-2, FCN2, newborn, prematurity, Immunologic diseases. Allergy, RC581-607
Abstract

Ficolin-2 is regarded as an important innate immunity factor endowed with both lectin (carbohydrate recognition) qualities and ability to induce complement activation. The aim of this study was to investigate the association of the FCN2 3’-untranslated region (3’UTR) polymorphisms with ficolin-2 expression and perinatal complications in preterm neonates. The sequencing analysis allowed us to identify six 3’UTR polymorphisms with minor allele frequency (MAF) >1%: rs4521835, rs73664188, rs11103564, rs11103565, rs6537958 and rs6537959. Except for rs4521835, all adhered to Hardy-Weinberg expectations. Moreover, rs6537958 and rs6537959 were shown to be in perfect linkage disequilibrium (LD) with nine other genetic polymorphisms: rs7040372, rs7046516, rs747422, rs7847431, rs6537957, rs6537960, rs6537962, rs11462298 and rs7860507 together stretched on a distance of 1242 bp and very high LD with rs11103565. The 3’UTR region was shown to bind nuclear extract proteins. The polymorphisms at rs4521835 and rs73664188 were found to influence serum ficolin-2 concentration significantly. All polymorphisms identified create (together with exon 8 polymorphism, rs7851696) two haplotype blocks. Among 49 diplotypes (D1-D49) created from rs7851696 (G>T), rs4521835 (T>G), rs73664188 (T>C), rs11103564 (T>C), rs11103565 (G>A) and rs6537959 (T>A), twenty two occurred with frequency >1%. Two diplotypes: D13 (GTTTGT/GGTCGT) and D10 (GTTTGT/GGTCGA), were significantly more frequent among preterm neonates with early onset of infection and pneumonia, compared with newborns with no infectious complications (OR 2.69 and 2.81, respectively; both p

Published
2021
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3. Association of the FCN2 Gene Promoter Region Polymorphisms with Very Low Birthweight in Preterm Neonates

Author

Agnieszka Szala-Poździej, Anna S. Świerzko, Gabriela Gajek, Maja Kufelnicka-Babout, Karolina Chojnacka, Paulina Kobiela, Dariusz Jarych, Katarzyna Sobczuk, Jan Mazela, Iwona Domżalska-Popadiuk, Jarosław Kalinka, Hideharu Sekine, Misao Matsushita, and Maciej Cedzyński

Subjects
Inorganic Chemistry, ficolin-2, FCN2, newborn, neonate, prematurity, single nucleotide polymorphism, very low birthweight, Organic Chemistry, General Medicine, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Catalysis, Computer Science Applications
Abstract

Single nucleotide polymorphisms (SNPs) localised to the promoter region of the FCN2 gene are known to influence the concentration of ficolin-2 in human serum and therefore potentially have clinical associations. We investigated the relationships between SNPs at positions −986 (A > G), −602 (G > A), −64 (A > C) and −4 (A > G) and clinical complications in 501 preterms. Major alleles at positions −986 and −64 and A/A homozygosity for both polymorphisms were less frequent among babies with very low birthweight (VLBW, ≤1500 g) compared with the reference group (OR = 0.24, p = 0.0029; and OR = 0.49, p = 0.024, respectively for A/A genotypes). A lower frequency of G/G homozygosity at position −4 was associated with gestational age

Published
2022
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4. Polymorphisms of the

Author

Anna S, Świerzko, Dariusz, Jarych, Gabriela, Gajek, Karolina, Chojnacka, Paulina, Kobiela, Maja, Kufelnicka-Babout, Mateusz, Michalski, Katarzyna, Sobczuk, Agnieszka, Szala-Poździej, Misao, Matsushita, Jan, Mazela, Iwona, Domżalska-Popadiuk, David C, Kilpatrick, Jarosław, Kalinka, Hideharu, Sekine, and Maciej, Cedzyński

Subjects
Male, Genotype, Immunology, prematurity, Infant, Newborn, Pneumonia, Infections, Polymorphism, Single Nucleotide, Immunity, Innate, Linkage Disequilibrium, 3’UTR, Gene Frequency, newborn, Lectins, ficolin-2, Humans, FCN2, Female, Genetic Predisposition to Disease, 3' Untranslated Regions, Complement Activation, Genetic Association Studies, Infant, Premature, Original Research
Abstract

Ficolin-2 is regarded as an important innate immunity factor endowed with both lectin (carbohydrate recognition) qualities and ability to induce complement activation. The aim of this study was to investigate the association of the FCN2 3’-untranslated region (3’UTR) polymorphisms with ficolin-2 expression and perinatal complications in preterm neonates. The sequencing analysis allowed us to identify six 3’UTR polymorphisms with minor allele frequency (MAF) >1%: rs4521835, rs73664188, rs11103564, rs11103565, rs6537958 and rs6537959. Except for rs4521835, all adhered to Hardy-Weinberg expectations. Moreover, rs6537958 and rs6537959 were shown to be in perfect linkage disequilibrium (LD) with nine other genetic polymorphisms: rs7040372, rs7046516, rs747422, rs7847431, rs6537957, rs6537960, rs6537962, rs11462298 and rs7860507 together stretched on a distance of 1242 bp and very high LD with rs11103565. The 3’UTR region was shown to bind nuclear extract proteins. The polymorphisms at rs4521835 and rs73664188 were found to influence serum ficolin-2 concentration significantly. All polymorphisms identified create (together with exon 8 polymorphism, rs7851696) two haplotype blocks. Among 49 diplotypes (D1-D49) created from rs7851696 (G>T), rs4521835 (T>G), rs73664188 (T>C), rs11103564 (T>C), rs11103565 (G>A) and rs6537959 (T>A), twenty two occurred with frequency >1%. Two diplotypes: D13 (GTTTGT/GGTCGT) and D10 (GTTTGT/GGTCGA), were significantly more frequent among preterm neonates with early onset of infection and pneumonia, compared with newborns with no infectious complications (OR 2.69 and 2.81, respectively; both p

Published
2021

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