1. Redox-State-Mediated Regulation of Cytochrome c Release in Apoptosis Revealed by Surface-Enhanced Raman Scattering on Nickel Substrates.
- Author
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Zhu J, Jiang M, Ma H, Zhang H, Cheng W, Li J, Cai L, Han XX, and Zhao B
- Subjects
- Binding Sites, Cardiolipins chemistry, Cardiolipins metabolism, Cytochromes c chemistry, HeLa Cells, Humans, Mitochondria drug effects, Mitochondria metabolism, Molecular Dynamics Simulation, Nanostructures chemistry, Nanostructures toxicity, Nickel chemistry, Oxidation-Reduction, Peroxidases metabolism, Reactive Oxygen Species metabolism, Spectrum Analysis, Raman, Apoptosis, Cytochromes c metabolism, Nickel metabolism
- Abstract
The interaction of cytochrome c (Cyt c) with cardiolipin (CL) is believed to play an important role in the initial events of apoptosis. Herein, we investigate the structural changes of CL-bound Fe
2+ Cyt c and the correlation with Cyt c release through surface-enhanced Raman spectroscopy (SERS) on nickel substrates. The SERS results together with molecular dynamics simulation reveal that Fe2+ Cyt c undergoes autoxidation and a relatively larger conformational alteration after binding with CL, inducing higher peroxidase activity of Cyt c and higher permeability of the CL membrane compared with those induced by the Fe3+ Cyt c. The proapoptotic activity and SERS effect of the Ni nanostructures allow the in situ study of the redox-state-dependent Cyt c release from isolated mitochondria, which reveals for the first time that the ferrous state of Cyt c most likely plays a more important role in triggering apoptosis., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)- Published
- 2019
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