1. Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical.
- Author
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Fernández ‐ de ‐ Alba, Carlos, Takahashi, Hiroki, Richard, Alexandre, Chenavier, Yves, Dubois, Lionel, Maurel, Vincent, Lee, Daniel, Hediger, Sabine, and De Paëpe, Gaël
- Subjects
POLARIZATION (Nuclear physics) ,NUCLEAR magnetic resonance spectroscopy ,LIPOSOMES ,LIPIDS ,BIRADICALS - Abstract
Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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