1. Nortriptyline protects testes against germ cell apoptosis and oxidative stress induced by testicular ischaemia/reperfusion.
- Author
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Yazdani I, Ghazi-Khansari M, Saeedi Saravi SS, Nobakht M, Majdani R, Rezayat SM, Mousavi SE, Yari A, and Dehpour AR
- Subjects
- Adrenergic Uptake Inhibitors administration & dosage, Animals, Caspase 3 metabolism, Catalase metabolism, Disease Models, Animal, Glutathione Peroxidase metabolism, Humans, In Situ Nick-End Labeling, Male, Malondialdehyde metabolism, Nortriptyline administration & dosage, Rats, Rats, Wistar, Reperfusion Injury etiology, Reperfusion Injury pathology, Sperm Count, Spermatic Cord Torsion pathology, Spermatozoa pathology, Superoxide Dismutase metabolism, Testis blood supply, Testis pathology, Adrenergic Uptake Inhibitors pharmacology, Apoptosis drug effects, Cytoprotection, Nortriptyline pharmacology, Oxidative Stress drug effects, Reperfusion Injury prevention & control, Spermatic Cord Torsion complications, Spermatozoa drug effects, Testis drug effects
- Abstract
We designed this experiment to evaluate the effects of nortriptyline on testicular injury after torsion/detorsion (T/D). Ninety-six adult Wistar rats were divided into six groups 16 each in control group (Group 1), sham operated (Group 2), T/D + saline (Group 3), and in groups 4-6; were administered 2, 10 and 20 mg kg
-1 , i.p. of nortriptyline 30 and 90 min after torsion respectively. Testicular torsion was created by twisting the right testis 720° in clockwise direction for 1 h. In six rats of each group, tissue MDA level and caspase-3 activity increased and the activities of catalase, superoxide dismutase and glutathione peroxidase decreased in compared with control group 4 h after detorsion (P < 0.001). In six rats of each group 24 h after detorsion, histopathological changes and germ cell apoptosis were significantly deteriorated by measuring mean of seminiferous tubules diameters (MSTD) and TUNEL test. Moreover, 30 days after T/D, sperm concentration and motility were examined in rest of rats. Pre- and post-reperfusion nortriptyline could reduce MDA and caspase-3 levels and normalise antioxidant enzymes activities, dose dependently. Germ cell apoptosis was significantly decreased, and the MSTD, as well as sperm functions, were significantly improved. Inhibition of mitochondrial permeability transition pore is probably involved in protective effects of nortriptyline against testicular T/D cell damages., (© 2016 Blackwell Verlag GmbH.)- Published
- 2017
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