1. HPV Genotyping of Modified General Primer-Amplicons Is More Analytically Sensitive and Specific by Sequencing than by Hybridization.
- Author
-
Meisal R, Rounge TB, Christiansen IK, Eieland AK, Worren MM, Molden TF, Kommedal Ø, Hovig E, Leegaard TM, and Ambur OH
- Subjects
- Adolescent, Adult, Chlamydia Infections, DNA, Viral genetics, Female, Genetic Variation, Genotyping Techniques methods, Humans, Hybridization, Genetic, Papillomavirus Infections diagnosis, Sensitivity and Specificity, Uterine Cervical Neoplasms diagnosis, Young Adult, DNA Primers genetics, High-Throughput Nucleotide Sequencing methods, Nucleic Acid Hybridization, Papillomaviridae genetics, Papillomavirus Infections virology, Uterine Cervical Neoplasms virology
- Abstract
Sensitive and specific genotyping of human papillomaviruses (HPVs) is important for population-based surveillance of carcinogenic HPV types and for monitoring vaccine effectiveness. Here we compare HPV genotyping by Next Generation Sequencing (NGS) to an established DNA hybridization method. In DNA isolated from urine, the overall analytical sensitivity of NGS was found to be 22% higher than that of hybridization. NGS was also found to be the most specific method and expanded the detection repertoire beyond the 37 types of the DNA hybridization assay. Furthermore, NGS provided an increased resolution by identifying genetic variants of individual HPV types. The same Modified General Primers (MGP)-amplicon was used in both methods. The NGS method is described in detail to facilitate implementation in the clinical microbiology laboratory and includes suggestions for new standards for detection and calling of types and variants with improved resolution., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2017
- Full Text
- View/download PDF