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Abstract: Synapsin 1 (SYN1) is a phosphoprotein involved in nerve signal transmission. The porcine SYN1 promoter orthologue was cloned and characterized to provide a means of expressing a transgene specifically in neurons. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. Expression analysis of SYN1 demonstrated presence of transcript during embryonic development. Analysis of GFP expression in transgenic zebrafish embryos suggests that the pig SYN1 promoter directs expression in neuronal cells. Thus, the SYN1 promoter is a good candidate for use in the generation of pig models of human neurodegenerative disorders. [Copyright &y& Elsevier]

Highlights: [•] Transcriptome sequencing yielded 223 mill porcine RNA-seq reads, and 59,000 transcribed locations. [•] Establishment of unique transcription profiles for ten porcine tissues including four brain tissues. [•] Comparison of transcription profiles at gene, isoform, promoter and transcription start site level. [•] Highlights a high level of regulation of neuro-related genes at both gene, isoform, and TSS level. [•] Our results emphasize the pig as a valuable animal model with respect to human biological issues. [ABSTRACT FROM AUTHOR]