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This paper is divided into two parts; the first describes a CFD analysis of a gland packing labyrinth seals in 20 MW steam turbine in LNG plant using ANSYS CFX code as an isothermal simulation in fluid domain. The results of two turbulence models k-ε and k-ω SST are compared and validated against experimental data. It is shown that the labyrinth seal leakage is proportional to the clearance cube so any variation in clearance can have a significant impact on the steam leakage. This clearance between the shaft and the labyrinth seal vary as a function of operating temperature due to the different rates of thermal expansion of the materials for these two components. Likewise the fluid temperature has a direct effect on fluid dynamic behavior. For that; the second part of the paper investigates the effect of fluid thermal conditions of the labyrinth seal via the heat transfer and Nusselt number variation in the stator and the rotor, for different cases of inlet temperature (400 °C, 200 °C, 100 °C, 50 °C) and pressure ratio (2.5, 4.5, 6.5) for an ideal gas. Also it is important to know the temperature distribution across tooth and cavities of the labyrinth seal, and finally the Influence of Inlet total temperature on fluid pressure drop in the labyrinth seal will be treated for improving and readjusting the steam turbine sealing system. [ABSTRACT FROM AUTHOR]