1. Mitochondrial <scp>DNA</scp> copy number and cytogenetic damage among fuel filling station attendants
- Author
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Panjakumar Karunamoorthy, Dhananjayan Venugopal, Riaz Mahmood, Ravichandran Beerappa, and Avinash S. Gaikwad
- Subjects
Male ,Mitochondrial DNA ,DNA Copy Number Variations ,Epidemiology ,Health, Toxicology and Mutagenesis ,Metabolite ,India ,010501 environmental sciences ,medicine.disease_cause ,DNA, Mitochondrial ,01 natural sciences ,Toxicology ,03 medical and health sciences ,chemistry.chemical_compound ,Occupational Exposure ,Biomonitoring ,Humans ,Medicine ,Benzene ,Genetics (clinical) ,Carcinogen ,030304 developmental biology ,0105 earth and related environmental sciences ,Chromosome Aberrations ,Pollutant ,0303 health sciences ,business.industry ,chemistry ,Case-Control Studies ,Micronucleus test ,Female ,business ,Gasoline ,Genotoxicity - Abstract
Fuel filling attendants are constantly exposed to the complex mixture of gasoline and all refinery environments are probably carcinogenic for humans. These workers are considered as an unorganized group in India and unaware of the risk. The present study was focused to monitor workplace pollutants (particulate matter size 10 [PM10 μm], total volatile organic compound [VOC], and carbon monoxide [CO]), benzene exposure (phenol), and to evaluate their genotoxicity effect with reference to relative mitochondrial DNA copy number (MtDNAcn), 8-OHdG (8-hydroxy-2'-deoxyguanosine), and micronuclei (MN) frequency (%) among fuel filling attendants. This study recorded 318 ± 134 and 1,050 ± 260 μg/m3 time-weighted average concentration of PM10 and CO, respectively. However, total VOC levels recorded were below the detectable level (BDL) to 290 ± 50 μg/m3 . A total of 53 subjects (26 exposed and 27 control) participated in this study with similar sociodemographic information. It was noticed that fuel filling attendants were not using proper personal protective equipment (PPE) and are younger generation. The significantly (p =
- Published
- 2020
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