1. A fluorescence turn-on detection of copper(II) based on the template-dependent click ligation of oligonucleotides.
- Author
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Wang F, Li Y, Li W, Chen J, Zhang Q, Anjum Shahzad S, and Yu C
- Subjects
- Alkynes chemistry, Ascorbic Acid chemistry, Azides chemistry, Cations, Divalent, Cycloaddition Reaction, Fluorescence, Humans, Limit of Detection, Molecular Probes chemical synthesis, Oligonucleotides chemical synthesis, Oxidation-Reduction, Spectrometry, Fluorescence, Staining and Labeling methods, Click Chemistry, Copper analysis, Molecular Probes chemistry, Oligonucleotides chemistry, Water chemistry, Water Pollutants, Chemical analysis
- Abstract
In this work, a fluorescence turn-on method for copper(II) detection is reported. A molecular beacon (MB) was designed as a template. Cu(2+) was reduced to Cu(+) in the presence of a reductant (ascorbic acid). Two short single-stranded oligonucleotides one was labeled with a 5'-alkyne and the other with 3'-azide group, proceeded a template-dependent chemical ligation through the Cu(I)-catalyzed azide-alkyne cycloaddition. The newly generated click-ligated long chain oligonucleotide, which was complementary to the MB, opened the MB hairpin structure and resulted in a turn on fluorescence. The increase in fluorescence intensity is directly proportional to the amount of Cu(2+) added to the assay solution. The present assay is quite sensitive and allows the detection of 2 nM Cu(2+). The described assay also exhibits high selectivity over other metal ions., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2015
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