1. Synthesis of Fucosyl-Oligosaccharides Using α-l-Fucosidase from Lactobacillus rhamnosus GG.
- Author
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Escamilla-Lozano Y, Guzmán-Rodríguez F, Alatorre-Santamaría S, García-Garibay M, Gómez-Ruiz L, Rodríguez-Serrano G, and Cruz-Guerrero A
- Subjects
- Bacterial Proteins isolation & purification, Bacterial Proteins metabolism, Cell Wall enzymology, Chromatography, High Pressure Liquid, Glycosides chemistry, Humans, Mass Spectrometry, Oligosaccharides chemistry, Prebiotics, Substrate Specificity, alpha-L-Fucosidase metabolism, Lacticaseibacillus rhamnosus enzymology, Oligosaccharides biosynthesis, alpha-L-Fucosidase isolation & purification
- Abstract
Fucosyl-oligosaccharides are natural prebiotics that promote the growth of probiotics in human gut and stimulate the innate immune system. In this work, the release of α-lfucosidase by Lactobacillus rhamnosus GG, and the use of this enzyme for the synthesis of fucosyl-oligosaccharides were investigated. Since α-lfucosidase is a membrane-bound enzyme, its release from the cells was induced by addition of 4-nitrophenyl-α-l-fucopyranoside ( p NP-Fuc). Enzyme activity associated with the cell was recovered at 78% of its total activity. Fucosyl-oligosaccharides where synthesized using α-l-fucosidase extract and p NP-Fuc as donor substrate, and D-lactose or D-lactulose as acceptor substrates, reaching a yield up to 25%. Fucosyllactose was obtained as a reaction product with D-lactose, and its composition was confirmed by mass spectrometry (MALDI-TOF MS). It is possible that the fucosyl-oligosaccharide synthesized in this study has biological functions similar to human milk oligosaccharides.
- Published
- 2019
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