1. High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis.
- Author
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Laremore TN, Ly M, Solakyildirim K, Zagorevski DV, and Linhardt RJ
- Subjects
- Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel economics, Nuclear Magnetic Resonance, Biomolecular, Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization, Electrophoresis, Polyacrylamide Gel methods, Heparin isolation & purification, Oligosaccharides isolation & purification
- Abstract
Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies., (Copyright (c) 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
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