1. Oocyte-specific deletion of N-WASP does not affect oocyte polarity, but causes failure of meiosis II completion.
- Author
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Wang, Zhen-Bo, Ma, Xue-Shan, Hu, Meng-Wen, Jiang, Zong-Zhe, Meng, Tie-Gang, Dong, Ming-Zhe, Fan, Li-Hua, Ouyang, Ying-Chun, Snapper, Scott B, Schatten, Heide, and Sun, Qing-Yuan
- Subjects
Reproductive Medicine ,Biochemistry and Cell Biology ,Biomedical and Clinical Sciences ,Biological Sciences ,Genetics ,Contraception/Reproduction ,1.1 Normal biological development and functioning ,Underpinning research ,Generic health relevance ,Actin-Related Protein 2-3 Complex ,Animals ,Cell Polarity ,Cytokinesis ,Female ,Male ,Meiosis ,Mice ,Mice ,Transgenic ,Microscopy ,Confocal ,Oocytes ,Signal Transduction ,Wiskott-Aldrich Syndrome Protein ,Neuronal ,cdc42 GTP-Binding Protein ,N-WASP ,oocyte ,polarity ,meiosis II ,cytokinesis ,Physiology ,Clinical Sciences ,Paediatrics and Reproductive Medicine ,Obstetrics & Reproductive Medicine ,Biochemistry and cell biology ,Reproductive medicine - Abstract
Study questionThere is an unexplored physiological role of N-WASP (neural Wiskott-Aldrich syndrome protein) in oocyte maturation that prevents completion of second meiosis.Summary answerIn mice, N-WASP deletion did not affect oocyte polarity and asymmetric meiotic division in first meiosis, but did impair midbody formation and second meiosis completion.What is known alreadyN-WASP regulates actin dynamics and participates in various cell activities through the RHO-GTPase-Arp2/3 (actin-related protein 2/3 complex) pathway, and specifically the Cdc42 (cell division cycle 42)-N-WASP-Arp2/3 pathway. Differences in the functions of Cdc42 have been obtained from in vitro compared to in vivo studies.Study design, samples/materials, methodsBy conditional knockout of N-WASP in mouse oocytes, we analyzed its in vivo functions by employing a variety of different methods including oocyte culture, immunofluorescent staining and live oocyte imaging. Each experiment was repeated at least three times, and data were analyzed by paired-samples t-test.Main results and the role of chanceOocyte-specific deletion of N-WASP did not affect the process of oocyte maturation including spindle formation, spindle migration, polarity establishment and maintenance, and homologous chromosome or sister chromatid segregation, but caused failure of cytokinesis completion during second meiosis (P
- Published
- 2016