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Oogenesis involves a sequence of cellular divisions and developmental changes leading to the formation of oocytes, whose role in development is to transfer genomic information to the next generation. During this process, the gene expression pattern changes considerably concomitant with genome remodeling, while genomic information is maintained. The development of the gonad in zebrafish is unique in that it goes through an initial ovarian phase and subsequently into either ovarian or testicular phases. How the germ cells choose to commit to an oogenic fate and enter meiosis or alternatively not to enter meiosis and commit to a spermatogenetic fate remains a key question in development. Lack of suitable markers has hampered the understanding of the principles controlling sex differentiation in zebrafish. The current study was aimed at finding substantive cytochemical markers to identify specific oocyte stages primarily focusing on the DNA and RNA component of cells, using fluorescent dyes: acridine orange and propidium iodide. The pattern of synthesis and appearance of nucleoli was stage specific and may be used to identify stages of oogenesis. A distinguishing and possibly diagnostic feature of the staining pattern observed was the low level of chromatin staining compared to other cellular structures. This may be related to the more diffuse state of chromatin that occurs prior to thickening of chromosomes from the pachytene stage onwards. Although the fluorescent dyes may be useful in determining the localization of nucleic acids in tissue sections, it was not possible to quantify the relative contribution of the DNA and RNA components of specific stages of oocyte growth., (Copyright © 2012 Elsevier GmbH. All rights reserved.)

Progression through mitosis and meiosis during early zebrafish ovarian development is accompanied by highly regulated series of transformations in the architecture of oocytes. These cytoskeletal-dependent membrane events may be assumed to be brought about by deployment of proteins. While the cytoskeleton and its associated proteins play a pivotal role in each of these developmental transitions, it remains unclear how specific cytoskeletal proteins participate in regulating diverse processes of oocyte development in zebrafish. Results from this study show that a pool of spectrin accumulates during oogenesis and parallels an increase in volume of oocytes at pre-vitellogenic stages of development. Spectrin labeling is restricted to the surface of oogonia, the cortex of post-pachytene oocytes and later accumulates on the cytoplasm of pre-vitellogenic and vitellogenic oocytes. Results here suggest a correlation between spectrin labeling, increased cytoplasm volume of oocytes, an increase in the number of nucleoli and accumulation of cytoplasmic organelles. Overall, these results suggest that synthesis and storage of spectrin during pre-vitellogenic stages of oogenesis primes the egg with a pre-established pool of membrane-cytoskeletal precursors for use during embryogenesis, and that the presence of spectrin at the oocyte sub-cortex is essential for maintaining oocyte structure., (Copyright © 2011 Elsevier GmbH. All rights reserved.)