1. Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains.
- Author
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Tian, Debin, Cao, Dianjun, Lynn Heffron, C., Yugo, Danielle M., Rogers, Adam J., Overend, Christopher, Matzinger, Shannon R., Subramaniam, Sakthivel, Opriessnig, Tanja, LeRoith, Tanya, and Meng, Xiang-Jin
- Subjects
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PORCINE reproductive & respiratory syndrome , *SWINE vaccination , *OPEN reading frames (Genetics) , *RESTRICTION fragment length polymorphisms , *SWINE genetics , *VACCINATION , *VIRUSES - Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of arguably the most economically important global swine disease. The extensive genetic variation of PRRSV strains is a major obstacle for heterologous protection of current vaccines. Previously, we constructed a panel of chimeric viruses containing only the ectodomain sequences of DNA-shuffled structural genes of different PRRSV strains in the backbone of a commercial vaccine, and found that one chimeric virus had an improved cross-protection efficacy. In this present study, to further enhance the cross-protective efficacy against heterologous strains, we constructed a novel chimeric virus VR2385-S3456 containing the full-length sequences of shuffled structural genes (ORFs 3-6) from 6 heterologous PRRSV strains in the backbone of PRRSV strain VR2385. We showed that the chimeric virus VR2385-S3456 induced a high level of neutralizing antibodies in pigs against two heterologous strains. A subsequent vaccination and challenge study in 48 pigs revealed that the chimeric virus VR2385-S3456 conferred an enhanced cross-protection when challenged with heterologous virus strain NADC20 or a contemporary heterologous strain RFLP 1-7-4. The results suggest that the chimera VR2385-S3456 may be a good PRRSV vaccine candidate for further development to confer heterologous protection. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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