1. Illuminating an Ecological Blackbox : Using High Throughput Sequencing to Characterize the Plant Virome Across Scales
- Author
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Maclot, François, Candresse, Thierry, Filloux, Denis, Malmstrom, Carolyn M., Roumagnac, Philippe, van der Vlugt, René, Massart, Sébastien, Université de Liège - Gembloux, Biologie du fruit et pathologie (BFP), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Bordeaux (UB), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Michigan State University [East Lansing], Michigan State University System, Wageningen University and Research [Wageningen] (WUR), and Fonds de la Recherche Scientifique – FNRS under Grant No. 1.1.309.19F06
- Subjects
Écologie ,F40 - Écologie végétale ,virus ecology and evolution ,[SDV]Life Sciences [q-bio] ,Virologie végétale ,Virus phytopathogène ,Relation hôte pathogène ,Review ,opportunities and challenges ,plant virome ,Microbiology ,F30 - Génétique et amélioration des plantes ,high throughput sequencing ,Interactions biologiques ,Biointeractions and Plant Health ,santé des plantes ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,historical advances ,pathologie végétale ,H20 - Maladies des plantes ,Virus des végétaux ,PE&RC ,[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy - Abstract
The ecology of plant viruses began to be explored at the end of the 19th century. Since then, major advances have revealed mechanisms of virus-host-vector interactions in various environments. These advances have been accelerated by new technlogies for virus detection and characterization, most recently including high throughput sequencing (HTS). HTS allows investigators, for the first time, to characterize all or nearly all viruses in a sample without a priori information about which viruses might be present. This powerful approach has spurred new investigation of the viral metagenome (virome). The rich virome datasets accumulated illuminate important ecological phenomena such as virus spread among host reservoirs (wild and domestic), effects of ecosystem simplification caused by human activities (and agriculture) on the biodiversity and the emergence of new viruses in crops. To be effective, however, HTS-based virome studies must successfully navigate challenges and pitfalls at each procedural step, from plant sampling to library preparation and bioinformatic analyses. This review summarizes major advances in plant virus ecology associated with technological developments, and then presents important considerations and best practices for HTS use in virome studies.
- Published
- 2020