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Zein-coated magnesium oxide nanoparticles (zMgO NPs) can potentially improve cement adaptation to the tooth-restoration interface, which would aid in minimizing marginal leakage and secondary caries. The aim of this study was to assess the effect of incorporating zMgO NPs on the adaptation of self-adhesive resin cement using cross-polarization optical coherence tomography (CP-OCT) and scanning electron microscopy (SEM). Resin inlays were fabricated to be cemented in Class-I cavities of extracted human molars. All specimens were randomly divided into five groups (n = 10), and the resin inlays were cemented using self-adhesive resin cement with various concentrations of zMgO NPs (0% [control], 0.3%, 0.5%, 1%, 2%). Characterization was done by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and SEM. The specimens were examined for interfacial adaptation under CP-OCT. Floor and wall adaptation measurements were analyzed by software on 20 B-scans, and samples were sectioned for interfacial measurement by SEM. Results for CP-OCT and SEM showed a statistically significant increase of adaptation in the floor and wall of resin cement filled with zMgO NPs compared to the control. The samples enhanced with 0.3% and 0.5% showed a statistically significantly better adaptation in floor and wall in CP-OCT and SEM. However, there was no significant difference between the 1%, 2%, and control groups for CP-OCT and SEM analysis. The incorporation of zMgO NPs in self-adhesive resin cement can enhance the cement's properties by significantly improving its wall and floor adaptation. • Nanoparticles can influence the mechanical properties of dental materials. • Better adaptation of resin cement to the tooth-restoration interface is desirable. • Zein-coated magnesium oxide nanoparticles could enhance the cement's adaptation. • Optical Coherence Tomography can measure gaps in composite restoration interfaces. • The modified resin cement shows significantly improved floor and wall adaptation. [ABSTRACT FROM AUTHOR]

The objective of this study was to assess and compare the internal adaptation of various pulp-capping materials, namely TheraCal, Biodentine, and mineral trioxide aggregate (MTA), on the dentin of permanent teeth through the utilization of micro-computed tomography (MCT) and optical coherence tomography (OCT). Thirty permanent molars were divided into three groups using a random process: group A (TheraCal), group B (Biodentine), and group C (MTA, which served as the control group). On the buccal surface of each tooth, a class V cavity of a standardized cylindrical shape was prepared. Subsequently, the respective pulp-capping material was applied to the cavity based on the assigned group, followed by restoration with composite resin. Based on the MCT results, it was observed that group A had a considerably larger gap volume in comparison to groups B and C (p < 0.001). There was no significant difference in gap volume between groups B and C. Regarding the OCT findings, group A displayed a substantially higher level of light reflection than groups B and C (p < 0.001). Group C exhibited a significantly lower level of light reflection in comparison to group B (p < 0.001). Biodentine and MTA revealed similar outcomes in terms of how well they adhered to the dentinal surface in permanent teeth. Both materials exhibited superior performance in comparison to TheraCal. The utilization of OCT in clinical practice could be advantageous as it enables dentists to monitor and evaluate restorations during post-treatment follow-up. It is imperative to intensify efforts aimed at making OCT equipment more accessible and applicable, overcoming its current limitations, and allowing for its widespread utilization in clinical practice. [ABSTRACT FROM AUTHOR]

Dental composite is the most used aesthetic restorative biomaterial worldwide. However, it undergoes polymerisation shrinkage that could lead to loss of the interfacial seal between tooth and resin in some circumstances. This demands high‐resolution imaging technologies to detect these defects. This study carried out a comparison between microcomputed tomography (micro‐CT; Shimadzu, Japan) and swept‐source optical coherence tomography (SS‐OCT; Santec, Japan) in the detection of marginal adaptation defects at the tooth‐resin interface. Unlike in micro‐CT, it was possible to outline interfacial gaps along with tooth‐resin interfaces with SS‐OCT, which was attributed to the Fresnel diffraction of light. This in vitro comparison demonstrates SS‐OCT has great potential in dental imaging to effectively assess dental composite adaptation and marginal defects when high resolution is desired in real time. Lay Description: Detection of tooth‐colored restoration defects had been assessed by different radiographic methods. However, most of these methods are either invasive or suffer from low‐resolution. In this study, a comparison has been carried out between two different high‐resolution imaging systems; microcomputed tomography and optical coherence tomography, to explore their potentials in detecting restorations defects. The results showed optical coherence tomography has a great accuracy in locating the underlying defects when the obtained images were validated against confocal laser scanning microscopy images. [ABSTRACT FROM AUTHOR]

Background. Orthodontic patients are at high risk to develop caries. This study is introducing a clinical method detecting interfacial defects between ceramic brackets and enamel utilizing optical coherent tomography in addition to using the nanoleakage expression in vitro test. Methods. Transbond XT primer and moisture insensitive primer (MIP) were bonded to 75 human premolar enamel surfaces and divided into (XTD), (MIPD), and (MIPW) groups. The (XTD) and (MIPD) groups had ceramic brackets bonded to dry enamel surfaces using TransBond and moisture insensitive primers, respectively, while the (MIPW) samples were bonded to moist enamel using moisture insensitive primer. All specimens were examined under crosspolarization optical coherence tomography. Debonding forces of the brackets to 45 teeth (15 teeth/group). 30 bonded specimens (15 specimens/group) were cross-sectioned to detect the nanoleakage expression using scanning electron microscope equipped with energy-dispersive spectroscopy (SEM/EDS). The degree of conversion of the specimens in the experimental groups was tested using attenuated total reflectance Fourier transform infrared spectroscopy (FTIR/ATR). Results. Optical coherence tomography detected the interfacial defects between the ceramic brackets and tooth structure. One way ANOVA showed that (XTD) and (MIPD) groups recorded significantly higher bond strength values and less nanoleakage expression when compared to MIPW (p > 0.05). Conclusions. Optical coherence tomography can be utilized to detect interfacial adhesive-tooth defects. Dry enamel surfaces improve the quality of the enamel/primer interface (200 words). [ABSTRACT FROM AUTHOR]

Despite the clinical appeal of restoring deep class II cavities in single increment using bulk-fill resin composite, sealing of bulk-filled composite restorations is a concern. This study evaluated interfacial adaptation of bulk-fill composite restoration to axial wall and gingival floor of class II cavities using cross-polarization optical coherence tomography (CP-OCT). Box-shaped class II cavities were prepared in extracted molars and divided into three groups (n = 7) according to adhesive used; Clearfil SE Bond 2 (SE2), Tetric-N Bond Self-Etch (TSE) or Tetric-N Bond Universal (TNU). All adhesives were applied in self-etch mode and according to manufacturers' recommendation. Then, preparations were bulk-filled with Filtek Bulk Fill Posterior Restorative resin composite and immersed in a contrast agent. Tomographic images of axial wall and gingival floor of each restoration were obtained by CP-OCT (IVS-300, Santec) with a central wavelength of 1330 nm and were imported to an image analysis software to quantify microleakage. Mann–Whitney U test showed statistically significant difference in microleakage percentage between the groups at both axial wall and gingival floor (p < 0.05). SE2 group had the lowest percentage of microleakage (p < 0.05), as only few cross-sections showed areas of reflections from contrast agent penetrating into axial wall (8.23 ± 6.8) and gingival floor (7.07 ± 4.1), followed by TNU group (18.13 ± 12.9 axially and 30.61 ± 11.9 gingivally). Microleakage was frequently observed at the axial wall and gingival floor of TSE group, showing the highest percentages of 25.50 ± 12.5 and 36.97 ± 10.2, respectively (p < 0.05). All tested groups exhibited different extent of interfacial microleakage, however, two-step self-etch adhesive yielded superior adaptation in comparison to one-step self-etch adhesive and universal adhesive. [ABSTRACT FROM AUTHOR]

Despite improvements in dental adhesive materials, internal adaptation remains a challenge in bonded restorations. The aim of this study was to compare microgaps and internal floor adaptation between two different self-etch adhesives in class-V cavities using cross-polarization optical coherence tomography (CP-OCT). In this in vitro study, standardized round class-V cavities were prepared in 20 non-carious human upper central incisor teeth. They were randomly divided into two groups, TN and SE, with each group receiving a different dental adhesive. In TN group, the adhesive used was all-in-one Tetric N-Bond Self-Etch (TN; Ivoclar/Vivadent, Liechtenstein), while SE group was bonded with two-step self-etch Clearfil SE Bond 2 adhesive (SE; Kuraray Noritake Dental Inc, Japan). The prepared cavities were restored with flowable composite and then stored in distilled water for 24 h. Next, they were immersed in silver nitrate, followed by immersion in a photo-developing solution. Optical comparison was carried out by CP-OCT to assess microgaps and composite adaptation at the cavity floor. A Mann–Whitney test was applied to the data, which showed a statistically significant difference in composite adaptation among the two groups (p < 0.001) with the SE group showing superior adaptation. CP-OCT is a reliable tool for non-invasive imaging that gives an insight into composite performance. Better adaptation was found with the two-step self-adhesive for the composite used in this study. [ABSTRACT FROM AUTHOR]

Objective: This study evaluated interfacial gap formation in bulk-fill composite restorations using cross-polarization optical coherence tomography (CP-OCT). Materials and methods: Two bulk-fill resin composite materials each with its specific adhesive were used; light-cured Filtek Bulk Fill Posterior Restorative with Scotchbond Universal (3M ESPE) "group A" and dual-cure bulk-fill composite BulkEZ with Prelude One (Danville Materials) "group B." Proximal cavities (2 × 2 × 4.5 mm; width × length × depth) prepared on extracted human molars (n = 5) were restored with adhesives applied in self-etch mode and the composite placed in bulk. After 24 h storage, the specimens were immersed in ammoniacal silver nitrate solution for 24 h followed by a photo-developing solution for 8 h. Later, CP-OCT (Santec, Japan) at 1310 nm center wavelength was used to scan each specimen. Interfacial gap at the gingival floor was analyzed on cross-sectional OCT images obtained from each specimen at 0.5 mm interval using ImageJ analysis software. Result: Mann–Whitney test showed a significant difference (p < 0.05) between the groups with group A showing higher percentage of gap formation at the gingival floor. Conclusion: Within the limitation of the study, a better sealing of gingival margin was achieved with the dual-cure bulk-fill dental composite compared to the light-cured composite. [ABSTRACT FROM AUTHOR]

To take full advantage of focused ion beam (FIB) in preparation of ultrathin sections of biological tissues, we have used a cryo-milling process. In this study, extracted human teeth were scanned by optical coherence tomography to inspect the samples for intactness and to determine the area of interest. Then, the selected area of interest was cross-sectioned for examination under a confocal laser scanning microscope to determine the target location of the dentinoenamel junction (DEJ) that was later milled by cryo-FIB at preset parameters, followed by transmission electron microscope examination of the final sliced specimens for ultrastructural characterization. The proposed technique was able to outline the DEJ and to identify the different tooth layers in a single section, without artefacts or tissue damage. The DEJ was outlined as fine longitudinal projections intermingling between the solid electron-dense enamel and intricate electron-lucent hollow dentin. In conclusion, this study has shown the great potential of cryo-FIB in handling different biological tissues having different physical properties, with great precision and accuracy and minimum artefacts. [ABSTRACT FROM AUTHOR]

Attrition and wear of tooth surface occur with aging and result in loss of enamel, with exposure and histological changes in dentin. Dealing with attrited teeth and restoration of the lost tissue are clinically challenging. The main objective of this study is to characterize the exposed transparent dentin in the occlusal surface of attrited teeth by optical coherence tomography (OCT). Naturally attrited, extracted human teeth with occlusal-transparent dentin were investigated in comparison to sound and carious teeth. The teeth were subjected to OCT imaging and then cross-sectioned and polished. OCT B-scans were compared to light microscopy images of the same cross section. In OCT images, some changes were evident at the transparent dentin in attrited teeth. An OCT attenuation coefficient parameter ( μ) was derived based on the Beer-Lambert law as a function of backscatter signal slope. The mean values of μ were 1.05 ± 0.3, 2.23 ± 0.4, and 0.61 ± 0.27 mm for sound, carious, and transparent dentins, respectively. One-way ANOVA with Tukey's post-hoc showed a significant difference between groups ( p < 0.05). Physiological changes in transparent dentin that involve deposition of mineral casts in the dentinal tubules lead to lower attenuation of OCT signal. OCT has a potential role to detect transparent dentin on the surface of attrited teeth and can be used in the future as a clinical adjunct tool. [ABSTRACT FROM AUTHOR]

Optical coherence tomography (OCT) is an interferometric imaging technique. This study aimed to employ OCT to evaluate four different resin-based materials including a coating containing glass-ionomer filler and calcium, a giomer, and twofluoride-releasing self-etch resins. The coating and its underlying and adjacent enamel were monitored using swept-source OCT (center wavelength: 1330 nm) at baseline, after 5,000 thermal cycles, and after 1, 4 and 7 days of demineralization (pH 4.5). The coatings showed different thicknesses (60-250 micrometers) and various levels of structural and interfacial integrity. OCT could detect a demineralization inhibition zone adjacent to the edge of the fluoride- and calcium-releasing material. Localized demineralization was occasionally observed under thinner coatings. Protection of susceptible enamel surfaces by thin resin-based bioactive coatings provides protection from demineralization. OCT can be used to non-destructively monitor the integrity of such coatings, as well as enamel changes beneath and adjacent to them. [ABSTRACT FROM AUTHOR]

Optical coherence tomography (OCT) is a potential clinical tool for enamel lesion monitoring. Swept-source OCT findings were compared with cross-sectional nanohardness findings of enamel. Subsurface bovine enamel lesions in three groups were subjected to (1) deionized water (control), (2) phosphoryl oligosaccharide of calcium (POs-Ca) or (3) POs-Ca with 1 ppm fluoride for 14 days. B-scans images were obtained at 1310-nm center wavelength on sound, demineralized and remineralized areas after 4, 7, and 14 days. The specimens were processed for cross-sectional nanoindentation. Reflectivity from enamel that had increased with demineralization decreased with remineralization. An OCT attenuation coefficient parameter (μt), derived based on the Beer-Lambert law as a function of backscatter signal slope, showed a strong linear regression with integrated nanohardness of all regions (p<0.001, r=-0.97). Sound enamel showed the smallest, while demineralized enamel showed the highest μt. In group three, μt was significantly lower at four days than baseline, but remained constant afterwards. In group two, the changes were rather gradual. There was no significant difference between groups two and three at 14 days in nanohardness or μt POs-Ca with fluoride-enhanced nanohardness of the superficial zone. OCT signal attenuation demonstrated a capability for monitoring changes of enamel lesions during remineralization. [ABSTRACT FROM AUTHOR]

Objectives: This study investigated class-I cavity floor adaptation by swept-source optical coherence tomography (OCT) in combination with microtensile bond strength (MTBS) using different filling methods. Methods: Two adhesive systems; Tokuyama Bond Force and Tri-S Bond Plus were used in conjunction with a universal composite (Estelite Sigma Quick) placed either incrementally (oblique) or in bulk with or without a flowable composite lining (Palfique Estelite LV). Ten serial B-scan images were obtained throughout each cavity by OCT (center wavelength: 1319 nm). In order to evaluate adaptation defined as the cavity floor percentage showing no gap, a significant increase in the signal intensity was considered as gap at the bonded interface of the cavity floor. The same specimens were then cut into beams to measure MTBS at the cavity floor. Results: Two-way ANOVA demonstrated that the interaction of adhesive systems and filling techniques was significantly affecting both adaptation and MTBS ( p < 0.05). There was a significant correlation between MTBS and adaptation at cavity floor ( p < 0.05). Cavity floor adaptation and MTBS were improved when incremental filling technique was applied, while the outcome of lining technique was variable. Conclusions: Quantitative assessment by OCT can non-destructively provide information on the performance and effectiveness of dental composites and restoration techniques. There was a moderate correlation between floor adaptation and bond strength in class-I cavities. Incremental application of composite restoration showed the best performance in terms of bond strength and internal adaptation. Clinical significance: Incremental application of composite restoration was the most advantageous placement technique in terms of bond strength and internal adaptation. The lack of placement pressure with flowable composites may affect their adaptation to all-in-one adhesives; therefore, the outcome of cavity lining by flowable composite was variable. [ABSTRACT FROM AUTHOR]

Abstract: Objectives: Regardless of the cause, gap formation at the tooth–restoration interface may result in treatment failure; non-destructive assessment and monitoring of these defects are important. The aim of this in vitro study is to assess the tooth–restoration interface using a non-invasive technique; swept source optical coherence tomography (SS-OCT) and to confirm the findings with confocal laser scanning microscope (CLSM). Methods: Cylindrical class-I cavities (3mm in diameter and 1.5mm in depth) were prepared in the occlusal surface of human premolars. Each cavity was restored using an all-in-one adhesive system (Clearfil Tri-S Bond) and one of the three types of composites placed in bulk; Majesty Posterior, AP-X and Majesty LV (all by Kuraray Medical, Japan). Ten serial cross-sectional images of the whole restored cavity were obtained by SS-OCT at 1319nm center wave length, to which locations the specimens were later trimmed, polished and observed under CLSM. An image analysis software was used to detect significant peaks in the signal intensity at the resin–dentin interface of the cavity floor. The presence and dimensions of gaps at the interface were also confirmed by CLSM. Results: Increased SS-OCT signal intensity along the interface corresponded well to the interfacial gaps detected by CLSM. The actual gap size detected ranged from 26μm to 1.9mm in length, and the universal composite APX showed lowest interfacial gaps. Conclusion: SS-OCT imaging technology can be used to non-invasively detect and quantify micrometer gaps at the bottom of composite restorations, and potentially become a monitoring tool for composite restorations both in the laboratory research, and in the clinics. [Copyright &y& Elsevier]

Objective. The aim of the present study was to evaluate the adaptation of newly introduced bioactive restorative materials to the cavity floor using cross-polarization optical coherence tomography (CP-OCT). Materials and Methods. Round class V cavities were prepared on the proximal surfaces of sixty non-carious human anterior teeth (0.5 mm depth × 4 mm diameter), which were divided into groups according to the restorative material (n = 15). In the VF group, Vertise flow composite (Kerr, Orange, CA, USA) was used, in the BF group, Beautifil II composite (Shofu, Koyoto, Japan) was used, and in the AB group, ACTIVA BioACTIVE composite (Pulpdent, Watertown, NY, USA) was used. Cavities were restored using the bulk filling technique and cured according to the manufacturers' instructions. Then, the specimens were immersed in a contrasting agent, and image acquisitions were taken by CP-OCT to calculate the adaptation percentage by using an image analysis software. Results. B-scans showed a diffuse bright band of white pixels at the tooth-resin interface that was interpreted as a micro-gap present between the cavity floor and restorative material. The Kruskal-Wallis test showed a statistically significant difference between all tested groups with the AB group representing the least gap formation, followed by the BF group, and then the VF group, which demonstrated the highest gap formation. Conclusions. In class V cavities, better adaptation to the cavity floor can be obtained when using ACTIVA BioACTIVE more than Vertise flow and Beautifil II composites. In addition, CP-OCT is considered a non-destructive imaging tool that helps in evaluating the quality of the tooth-restoration interface when bioactive composites are used. [ABSTRACT FROM AUTHOR]

Objective: In this in vitro study, cross-polarization optical coherence tomography (CP-OCT) was used to evaluate microleakage in dental composite restorations bonded to enamel with two types of one-step self-etching (SE) adhesives. Background data: One-step SE adhesives were proposed to simplify bonding of composite restorations. However, bonding of these simplified adhesive to dental enamel is still questionable. OCT is a promising diagnostic tool that allows micron-scale imaging of biological tissues and structures. Methods: Class-V cavities (4-mm diameter × 1-mm depth) were prepared on the labial surfaces of extracted human anterior teeth with margins and floor located in enamel. The cavities were then divided into two groups (n = 5) and restored with either Tetric N-Bond Universal (Ivoclar Vivadent, Liechtenstein) or Palfique Bond (Tokuyama Dental Corporation, Japan), followed by Estelite Flow Quick flowable composite (Tokuyama Dental Corporation). After storage in 100% humidity for 24 h, specimens were immersed in ammoniacal silver nitrate solution for another 24 h. Later, they were rinsed and immersed in photo-developing solution under fluorescent light for 8 h. CP-OCT at 1310 nm center wavelength was used to scan seven two-dimensional cross-sectional images from each specimen. Each cross-sectional image was analyzed using ImageJ software to quantify interfacial microleakage at the cavity floor. Results: Student's t-test showed significant difference between the tested adhesives (p < 0.05). Palfique Bond adhesive presented inferior interfacial adaptation as it obtained higher values of floor microleakage compared to Tetric N-Bond Universal adhesive group. Conclusions: CP-OCT enabled nondestructive quantitative assessment of microleakage in enamel dental restorations. [ABSTRACT FROM AUTHOR]