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BACKGROUND Oral epithelial dysplasia (OED) is the presence of cells of an abnormal type within a tissue, which may signify a stage preceding the development of cancer. Our aim was to determine the interrelation between the expression of multiple molecular markers and the histological features of oral dysplasia. METHODS Fifteen samples of OED (five for each severity degree) were analyzed through software assisted image cytometry nuclear morphology. p53 (wildtype and mutated form), Bax and Bcl2 expression was immunohistochemically determined, and the gene expression of MMP1, MMP2,MMP9 and hTERT was determined by RT-PCR. The mean, standard deviation, ANOVA and Fisher´s exact test (p

BACKGROUND Odontomas are odontogenic tumors with hamartoma features that are classified as compound or complex. Our objective was to characterize the proliferation of ectodermal and ectomesenchymal profile markers of primary cell cultures of complex and compound odontomas. METHODS Four samples of compound odontomas (OdCm) and three of complex odontomas (OdCx) were obtained from patients attending the Oral Pathology and Medicine Clinic of the Graduate Dental School, National Autonomous University of Mexico for primary culture generation. MTT, immunocytochemistry and RT-PCR assays of CD34, Sox2, Amel, Ambn, p21, EDAR, Msx1, Msx2, Pax9, RUNX2, BSP, OPN, Barx1 and GAPDH (control) were performed. Additionally, six paraffin-embedded odontomas were obtained for immunocytochemistry and RT-PCR validation assays. The mean and standard deviation were determined, and ANOVA and Kruskall-Wallis tests were performed. RESULTS Cultured compound odontoma exhibited higher proliferation, and an ectomesenchymal immunocytochemistry profile with predominant expression of Amel, BSP, Pax9, EDAR, Barx and Msx2; in complex cultured odontoma Sox2, CD34, RUNX2 and OPN predominated. Our statistical analysis showed a significant difference in PCR analysis (P

Objective To present a primitive monophasic sarcoma of the mandible in a young male with spindle-cell predominant component and non-specific immunohistochemical phenotype. Uncertain origin sarcomas are a very complex and heterogeneous group of neoplasms, categorized by their undetermined histogenesis and peculiar morphological features; generally associated to a specific translocation. This type of sarcomas has not sufficient clinical and diagnostic information because its low frequency in this site. Case report A 23-year-old male presented with slow-progressive mobility and tooth loss associated with gingival enlargement in the third quadrant. Local examination showed a firm mass of approximately 5 × 4 cm in the body of the mandible. X-ray examination revealed an extensive ill-defined radiolucency on the left side of the mandibular body. CT-scan assessed bone and soft tissue infiltration. Hemimandibulectomy was performed and gross examination consisted of a solid nodular mass. Microscopically a monotonous spindle-cell lesion with fascicular pattern was observed, hypercellular areas and hemangiopericytoid-like vessels were the predominant component. The neoplastic cells displayed pleomorphic nuclear features with vesicular chromatin. A wide immuhohistochemical panel was performed showing reactivity for Calponin, CD68, CD99, Bcl-2, TLE-1, NSE, FLI-1, WT1, PTEN and 40% Ki67 index. Conclusion Despite of the high sensitivity, antibodies are not conclusive for an accurate diagnosis. Molecular techniques were required to establish this entity with great implication in patient management. Complete assesment of undifferentiated sarcomas should include clinical/radiological correlation, histopathology, immunohistochemical staining and genetic confirmation.

Excessive fluoride ingestion has been identified as a risk factor for fluorosis and oxidative stress. The oxidative stress results from the loss of equilibrium between oxidative and antioxidative mechanisms that can produce kinase activation, mitochondrial disturbance and DNA fragmentation, resulting in apoptosis. Actually many people are exposed to no-adverted fluoride consumption in acute or chronic way. The aim of this study was to determine the effect of sodium fluoride on first molar germ in relation to its effect on antioxidative enzymes immunoexpression and apoptosis. Thirty first molar germs from 1-day-old Balb/c mice were cultured for 24 h with sodium fluoride (0 mM, 1 mM and 5 mM). Immunoexpression determination of CuZnSod, MnSod, catalase, Bax, Bid, caspase 8, caspase 9, caspase 3 and TUNEL assay were performed. Cellular disorganization in ameloblast and odontoblast-papilla zones was observed. CuZnSod and MnSod immunoexpression decrease in experimental groups. Caspase 8, caspase 3, Bax, Bid increase expression and more TUNEL positive cells in both experimental groups than control, suggest that apoptosis induced by fluoride is related to oxidative stress due to reduction of the enzymatic antioxidant.

P127. Expression of erbB, ERK, AKT, c-Fos, c-Jun and NF-kb in oral carcinogenesis golden Syria hamster model with 7,12-dimethylbenz(a)anthracene and alcohol L.-F. Jacinto-Aleman *, M.-D. Jimenez-Farfan , E.-R. Leyba-Huerta , A. Garcia-Carranca , J.-C. Hernandez-Guerrero a Universidad Nacional Autonoma de Mexico, Facultad de Odontologia, DEPeI, Mexico Universidad Nacional Autonoma de Mexico, Instituto Investigaciones Biomedicas, Mexico c Instituto Nacional de Cancerologia, Mexico