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Phytase producing strain of Klebsiella sp. DB-3 FJ711774.1 was isolated from poultry field soil. The bacteria produced extracellular phytase under submerged fermentation (Smf) using orange peel and other agro-residues as nutritive substrates. Among different substrates, orange peel flour and wheat bran (2% w/v) provide a maximal yield of production of phytase i.e., 3.15 and 2.41 U/ml respectively when Smf was carried out by using fermentative media supplemented with 0.2% sucrose, 0.1% NH4H2PO4; inoculated with 1.5% cell suspension 24 h age and then, incubated at 50oC for 72 h. As such, there is no report of phytase production by using orange peel as substrate. The phytate biosynthesis by bacteria cultivation on medium based on orange peel flour was accompanied by the liberation of soluble phosphate without any extra supplementation of metal ions. Based on these results the use agro residues in the form of orange peel flour could be one of the best and cost effective alternatives to the costly pure phytate for industrial production of phytase using selected strain of Klebsiella spp. [ABSTRACT FROM AUTHOR]

Statistical experimental designs were applied for the optimization of phytase production by a bacteria Klebsiella sp. DB3 in orange peel flour. Four factors i.e., orange peel flour, ammonium dihydrogen phosphate, temperature and pH were optimized using central composite design (CCD). The optimum levels of variables that supported maximum enzyme activity were orange peel bran 2.0%, sucrose 2.0% and ammonium dihydrogen phosphate (NH4H2PO4) 0.1% and pH 5.5. The validity of the model in optimized conditions was verified. An overall 5.25-fold enhancement in phytase activity (0.60 to 3.15 U) was attained due to the optimization. [ABSTRACT FROM AUTHOR]