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In the brackish water sedimentary environment of the Junggar Basin in Xinjiang, the organic-rich shale and shale oil formation with relatively low maturity, is characterized by relatively high abundance of C 28 regular steranes. However, the biogenic source of organic matter and favorable sedimentary environment for the type of source rock has been thoroughly classified. In this paper, organic geochemical and petrological, analyses of the Lucaogou Formation shale in the Jimusaer Sag, Junggar Basin in the north of Xinjiang Uygur Autonomy Region were carried out. The source rocks in the Lucaogou Formation are mainly composed of two types of maceral including lamalginite (cyanobacteria) and telalginite (green algae), with the former predominantly fueling the high C 28 regular sterane content. Petrological, organic and inorganic geochemical analyses suggest that the lamalginite was developed in low-salinity water while the telalginite was developed in high-salinity water. Based on geochemical and organic petrological characteristics, both the lamalginite and telalginite have good hydrocarbon-generating potential, but in the lower thermal evolution stage of the Lucaogou Formation source rock, telalginite have higher hydrocarbon-generating rate. • Lamalginite (cyanobacteria) is the primary source of C 28 regular steranes. • The lamalginite (cyanobacteria) is more developed when the salinity is low and the telalginite (green algae) is more developed when the salinity is high. • In the lower thermal evolution stage of the Lucaogou Formation source rock, the lamalginite (cyanobacteria) has lower hydrocarbon-generating rate than the telalginite (green algae). [ABSTRACT FROM AUTHOR]

• Sedimentary conditions in the Lucaogou Formation source rocks are described. • The telalginite generated hydrocarbons earlier than the lamalginite. • New parameter index to identify effective source rocks is proposed. The Middle Permian Lucaogou Formation in the Jimusaer Sag is a hotspot for exploring and developing lacustrine tight oil in the Junggar Basin, NW China. In this study, we evaluated the tight oil potential from the perspective of source rock hydrocarbon generation and expulsion. Based on a detailed organic, petrological, and geochemical characterization of the target interval within the key well JHBE, kinetic experiments were performed on representative shale samples from the well JHBE and the threshold of hydrocarbon expulsion of the source rock was established. The findings of this study revealed that the Lucaogou Formation shales were deposited in a dysoxic to anoxic and clay-poor lacustrine environment with variable salinity and their organic matter (OM) was contributed from both lamalginite and telalginite with minor vitrinite and inertinite, resulting in good to excellent source rock potential. The lamalginite was deposited in water with low salinity, whereas the telalginite developed in water with relatively higher salinity. Although the greater contribution of lamalginite resulted in a higher OM content than that of telalginite, the former generated a lower amount of hydrocarbons than the latter, because telalginite is capable of generating hydrocarbons earlier than lamalginite, which is indicated by the higher HCI (Hydrocarbon index, S 1 × 100/TOC), EOM/TOC (extraction of organic matter/total organic carbon), C 29 ββ/(αα + ββ), and C 29 ααα20S/(20S + 20R) sterane values for telalginite than for lamalginite and is also evident from the difference in activation energy distributions between lamalginite and telalginite source rocks. As determined from the relationships of sterane maturity parameters C 29 ββ/(αα + ββ) and C 29 ααα20S/(20S + 20R) vs EOM/TOC ratio and HCI values, the lower C 29 ββ/(αα + ββ) and C 29 ααα20S/(20S + 20R) limits for active source rocks were approximately 0.28 and 0.44, respectively. [ABSTRACT FROM AUTHOR]