1. Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart.
- Author
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Polancec D, Zenic L, Hudetz D, Boric I, Jelec Z, Rod E, Vrdoljak T, Skelin A, Plecko M, Turkalj M, Nogalo B, and Primorac D
- Subjects
- Adipocytes drug effects, Adipocytes immunology, Adventitia drug effects, Cartilage drug effects, Cell Differentiation drug effects, Endothelial Cells drug effects, Endothelial Cells immunology, Female, Flow Cytometry, Humans, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells immunology, Osteoarthritis immunology, Osteoarthritis metabolism, Pericytes drug effects, Pericytes immunology, Adventitia immunology, Cartilage metabolism, Immunophenotyping, Osteoarthritis drug therapy
- Abstract
Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF-LA and SVF-MLA samples, the following population phenotypes were identified within the CD45
- fraction: CD31+ CD34+ CD73± CD90± CD105± CD146± endothelial progenitors (EP), CD31+ CD34- CD73± CD90± CD105- CD146± mature endothelial cells, CD31- CD34- CD73± CD90+ CD105- CD146+ pericytes, CD31-CD34+ CD73± CD90+ CD105-CD146+ transitional pericytes, and CD31- CD34+ CD73high CD90+ CD105- CD146- supra-adventitial-adipose stromal cells (SA-ASC). The immunophenotyping profile of SVF-MLA was dominated by a reduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte-primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated., Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.- Published
- 2019
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