1. MicroRNA-21 regulates proliferation and differentiation of osteoclasts in periodontitis.
- Author
-
Xu Nuo, Cao Zhen, Li Xiaojie, and Shi Chun
- Subjects
- *
OSTEOCLASTOGENESIS , *OSTEOCLASTS , *MESSENGER RNA , *PERIODONTITIS - Abstract
BACKGROUND: MicroRNA-21 (miR-21) is a regulator of osteoclastogenesis and a promoter of osteoclast differentiation, but its role in periodontitis remains unclear. OBJ ECTlVE: To investigate whether miR-21 is involved in bone destruction in periodontitis. METHODS: Real-time PCR was used to detect and analyze the differential expression of miR-21 in periodontitis samples. Using liposome transfection method, miR-21 mimics (up-regulating miR-21) or miR-21 inhibitor (down-regulating miR-21) was used to transfect osteoclasis. Expressions of miR-21 and bone destruction markers TRAP and CTSK were detected by real-time PCR. Cell counting kit-8 was used to detect the miR-21 effect on osteoclast proliferation. RESULTS AND CONCLUSION: (1) MiR-21 expression increased in periodontitis samples. (2) When miR-21 mimics was transfected into osteoclasis, miR-21, TRAP and CTSK mRNA expression increased; when miR-21 inhibitor was transfected into osteoclasis, miR-21, TRAP and CTSK mRNA expression decreased. (3) Transfection with miR-21 mimics promoted the proliferation of osteoclasis, while transfection with miR-21 inhibitor inhibited the proliferation of osteoclasis. To conclude, miR-21 can be used as an important target for the treatment of periodontitis. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF