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The autonomic innervation of the ovary was studied in 12 mammalian species utilizing the cholinesterase method in combination with pseudocholinesterase inhibition for the cholinergic component, and glyoxylic acid histochemistry together with fluorometric determination of noradrenaline for the adrenergic component. Ovaries from cow, sheep, cat, and guinea pig were very richly supplied with adrenergic nerves in the cortical stroma, particularly enclosing follicles in various stages of development. In the follicular wall the nerve terminals were located in the theca externa, where they ran parallel to the follicular surface. Numerous adrenergic terminals also surrounded ovarian blood vessels. The adrenergic innervation was of intermediary density in the human ovary and in the pig, dog, cat, and opossum. Ovaries from rabbit, mouse and hamster had a sparse adrenergic nerve supply. The amount of intraovarian adrenergic nerves agreed well with the tissue concentration of noradrenaline in the various species. The cholinergic innervation was generally less well developed, but had the same distribution as the adrenergic system around blood vessels and in the ovarian stroma, including follicular walls.

The autonomic nerve supply of the guinea-pig ovary was investigated by a combination of light- and electron microscopy. At the light-microscopic level, adrenergic fibres were identified due to their formaldehyde-induced fluorescence. In addition, the ovary contained acetylcholinesterase-positive fibres. In all parts of the ovary, the adrenergic fibres were most numerous. At the ultrastructural level it was possible to identify the adrenergic nerve terminals with the aid of the false adrenergic transmitter, 5-hydroxy-dopamine. Thus, large numbers of adrenergic terminals, characterized by their content of 50-60 nm, electron-dense synaptic vesicles, were seen within the interstitial gland, where they formed close contacts with the endocrine cells (membrane-to-membrane distance, 20-100 nm). The follicular theca externa was also richly supplied by adrenergic nerves. At this location, close contacts (50-100 nm) were identified between the nerve terminals and the smooth muscle-like cells. Very few adrenergic nerve fibres were present in the theca interna of follicles or in the corpus luteum. Non-adrenergic nerve terminals, characterized by electron-lucent synaptic vesicles of 50-60 nm diameter, were observed together with the adrenergic fibres. They were always present in much lower numbers than the latter. No "p-type" nerves were identified by electron microscopy.

Fluorescence microscopy has shown that the guinea pig ovary contains not only vasomotor adrenergic nerves but also many nerve terminals running within endocrine structures. Electron microscopy of the interstitial gland confirmed the presence of adrenergic nerves distinguished by administration of the false transmitter, 5-hydroxydropamine. Axon varicosities, denuded of their Schwann cell ensheathing, innervated the endocrine cells in the interstitial gland, with a synaptic distance of approximately 400 A. This suggests that the interstitial gland participates in a neuroendocrine transducer mechanism.