1. Hydroxycinnamic acids in Crepidiastrum denticulatum protect oxidative stress-induced retinal damage.
- Author
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Ahn HR, Lee HJ, Kim KA, Kim CY, Nho CW, Jang H, Pan CH, Lee CY, and Jung SH
- Subjects
- Animals, Apoptosis drug effects, Caffeic Acids administration & dosage, Caffeic Acids analysis, Chlorogenic Acid administration & dosage, Chlorogenic Acid analogs & derivatives, Chlorogenic Acid analysis, Disease Models, Animal, Glaucoma etiology, Glaucoma prevention & control, Lipid Peroxidation drug effects, Male, Mice, Mice, Inbred ICR, N-Methylaspartate administration & dosage, Nerve Crush, Neuroprotective Agents, Optic Nerve, Plant Extracts administration & dosage, Rats, Rats, Sprague-Dawley, Retinal Diseases etiology, Succinates administration & dosage, Succinates analysis, Asteraceae chemistry, Coumaric Acids administration & dosage, Coumaric Acids analysis, Oxidative Stress, Retinal Diseases prevention & control
- Abstract
We investigated the effects of an ethanol extract of C. denticulatum (EECD) in a mouse model of glaucoma established by optic nerve crush (ONC), and found that EECD significantly protected against retinal ganglion cell (RGC) death caused by ONC. Furthermore, EECD effectively protected against N-methyl-d-aspartate-induced damage to the rat retinas. In vitro, EECD attenuated transformed retinal ganglion cell (RGC-5) death and significantly blunted the up-regulation of apoptotic proteins and mRNA level induced by 1-buthionine-(S,R)-sulfoximine combined with glutamate, reduced reactive oxygen species production by radical species, and inhibited lipid peroxidation. The major EECD components were found to be chicoric acid and 3,5-dicaffeoylquinic acid (3,5-DCQA) that have shown beneficial effects on retinal degeneration both in vitro and in vivo studies. Thus, EECD could be used as a natural neuroprotective agent for glaucoma, and chicoric acid and 3,5-DCQA as mark compounds for the development of functional food.
- Published
- 2014
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