1. Effects of phonophoresis with gold nanoparticles on oxidative stress parameters in a traumatic muscle injury model.
- Author
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Silveira PC, Victor EG, Notoya Fde S, Scheffer Dda L, Silva Ld, Cantú RB, Martínez VH, de Pinho RA, and Paula MM
- Subjects
- Animals, Antioxidants metabolism, Catalase metabolism, Dimethyl Sulfoxide metabolism, Disease Models, Animal, Glutathione Peroxidase metabolism, Lipid Peroxidation drug effects, Male, Muscle, Skeletal metabolism, Muscular Diseases metabolism, Phonophoresis methods, Rats, Rats, Wistar, Superoxide Dismutase metabolism, Thiobarbituric Acid Reactive Substances metabolism, Wounds and Injuries metabolism, Gold pharmacology, Metal Nanoparticles administration & dosage, Muscle, Skeletal drug effects, Muscular Diseases drug therapy, Oxidative Stress drug effects, Wounds and Injuries drug therapy
- Abstract
The aim of this study was to evaluate the effects of therapeutic pulsed ultrasound with gold nanoparticles on oxidative stress parameters after traumatic muscle injury in Wistar rats. The animals were randomly divided into nine groups (n = 6 each): sham (uninjured muscle); muscle injury without treatment; muscle injury and treatment with dimethyl sulfoxide (15 mg/kg); muscle injury and treatment with gold nanoparticles (27 µg); muscle injury and treatment with dimethyl sulfoxide + gold nanoparticles (Plus); muscle injury and therapeutic pulsed ultrasound; muscle injury and therapeutic pulsed ultrasound + dimethyl sulfoxide; muscle injury and therapeutic pulsed ultrasound + gold nanoparticles; and muscle injury and therapeutic pulsed ultrasound + Plus. Gastrocnemius injury was induced by a single-impact blunt trauma. Therapeutic pulsed ultrasound (6-min application, frequency 1.0 MHz, intensity 0.8 W/cm(2)) was used 2, 12, 24, and 48 h after trauma. Mitochondrial superoxide generation, lipid peroxidation, and protein carbonylation, and the activities of superoxide dismutase, glutathione peroxidase, and catalase were evaluated. The increase in the superoxide production and TBARS and carbonyl levels observed in the control group after muscle damage were reduced in animals exposed to therapeutic pulsed ultrasound plus nanoparticles. Similarly, antioxidants enzymes showed a decreased activity with the same treatment. Our work suggest that therapeutic pulsed ultrasound + dimethyl sulfoxide + gold nanoparticles has beneficial effects on the muscle healing process by inducing a decrease in oxidative stress parameters and most likely decreasing the deleterious effects of the inflammatory response.
- Published
- 2016
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