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Present work evaluates the salt tolerance of 29 domestic and foreign samples of millet (Panicum miliaceum L.) for identification of valuable genotypes for further use in the breeding programs. The study aimed to estimate the salt tolerance by screening the collection of millet samples at the germination stage of ontogenesis based on the changes of morphometric indicators for forecasting the reaction of genotypes to salinization. The salt stress inhibited the growth of sprouts and roots of millet samples. Unequal effect of salt stress onto the length of sprouts and roots of seedlings was noted. The samples Aktyubinskoye kormovoye, K-9681, Shortandinskoye-10, and Yarkoye-7 demonstrated an insignificant decrease of sprouts length in comparison with the control. The cultivar Saratovskoye-6 (standard) showed about a 50% decrease of sprouts length at 75 and 100 mm of NaCl, and to 70% decrease at 150 mm of NaCl. At all salinity concentrations, the smallest length of germinal roots of 7-day seedlings was noted at samples Yarkoye-5 and Pavlodarskoye and the greatest length of sprouts at samples K-9681, Yarkoye-5, Pavlodarskoye, Shortandinskoye-10 and Aktyubinskoye kormovoye. [ABSTRACT FROM AUTHOR]

Proso millet (Panicum miliaceum L.) is important in some Kazakhstan food, this research objective was to develop prebreeding waxy proso millet resource for breeding new varieties by using phenotyping and marker-assisted selection. The amylose content of the endosperm starch in a collection of 18 proso millet cultivars widely used in northern Kazakhstan were evaluated and ranged from 14.6% to 34.8%. The introduced glutinous millet accessions of PI436626 (Lung Shu 18), PI436625 (Lung Shu 16) were selected to cross with local cultivars (Saratovskoe 6 and Pamyati Bersieva). The potassium iodide (KI) staining method and PCR analysis using the 9bF/15delRB primer were used to identify the waxy trait for selection of the F2-F3 generation. Seeds staining dark blue to black were scored as wild type, while waxy seeds were stained as pinkish or amber to reddish-brown color. The hybridological analysis of inheritance of the "waxy" trait suggested that the segregation ratios for waxy endosperm in the F2 and F3 populations was close to 15:1 of the caryopses with blue and brown colors. PCR analysis with the 9bF/15delRB primer confirmed that this specific primer flanks a 123 bp fragment in amylose samples, while in glutinous samples, this primer promotes the amplification of 108 bp long PCR product (15 nucleotides deletion). Finally, the waxy lines were obtained and could be used for further testing. [ABSTRACT FROM AUTHOR]