1. Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics
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Allison Golden, Rebecca Barney, John Rek, Harriet Adrama, Andrew Rashid, Stephane Proux, François Nosten, Chris Lyman, Bryan Greenhouse, Dionicia Gamboa, Mallika Imwong, Maria Kahn, Michael Kalnoky, Warat Haohankhunnatham, Emmanuel Arinaitwe, Gonzalo J. Domingo, Maxwell Murphy, Ihn Kyung Jang, and Abby Tyler more...
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Plasmodium ,030231 tropical medicine ,Plasmodium vivax ,Antigens, Protozoan ,Biology ,Medical and Health Sciences ,Sensitivity and Specificity ,Human Malaria Array ,03 medical and health sciences ,chemistry.chemical_compound ,Rare Diseases ,0302 clinical medicine ,Malaria Rapid Diagnostics ,Diagnostic Tests ,Antigen ,Species Specificity ,Tropical Medicine ,Virology ,Lactate dehydrogenase ,parasitic diseases ,medicine ,Humans ,Routine ,Malaria antigen ,Multiplex ,Antigens ,Diagnostic Tests, Routine ,Diagnostic test ,DNA ,Articles ,DNA, Protozoan ,biology.organism_classification ,medicine.disease ,Malaria ,Vector-Borne Diseases ,Good Health and Well Being ,Infectious Diseases ,Quantifying Antigens ,chemistry ,Protozoan ,Asymptomatic malaria ,Parasitology ,Infection ,Multiplex Polymerase Chain Reaction ,purl.org/pe-repo/ocde/ford#3.03.06 [https] - Abstract
Author(s): Jang, Ihn Kyung; Tyler, Abby; Lyman, Chris; Rek, John C; Arinaitwe, Emmanuel; Adrama, Harriet; Murphy, Maxwell; Imwong, Mallika; Proux, Stephane; Haohankhunnatham, Warat; Barney, Rebecca; Rashid, Andrew; Kalnoky, Michael; Kahn, Maria; Golden, Allison; Nosten, Francois; Greenhouse, Bryan; Gamboa, Dionicia; Domingo, Gonzalo J | Abstract: Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development and screen asymptomatic malaria, we developed and evaluated the Q-Plex™ Human Malaria Array (Quansys Biosciences, Logan, UT), which quantifies the antigens commonly used in RDTs-Plasmodium falciparum-specific histidine-rich protein 2 (HRP2), P. falciparum-specific lactate dehydrogenase (Pf LDH), Plasmodium vivax -specific LDH (Pv LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction-confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. P. falciparum culture strains and samples from Peru indicated that HRP2 and Pf LDH combined improves detection of P. falciparum parasites with hrp2 and hrp3 deletions. This array can be used for antigen-based malaria screening and detecting hrp2/3 deletion mutants of P. falciparum. more...
- Published
- 2020
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