1. Limited movement of Cucumber mosaic virus (CMV) in yellow passion flower in Brazil
- Author
-
Gioria, R., Espinha, L. M., Rezende, JAM, Gaspar, J. O., Kitajima, E. W., Universidade de São Paulo (USP), and Universidade Estadual Paulista (Unesp)
- Subjects
PTA-ELISA ,PCR ,P. edulis f. flavicarpa ,Western blot ,dot-blot hybridization - Abstract
Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:13:17Z No. of bitstreams: 1 WOS000175209200002.pdf: 505294 bytes, checksum: 5d5013a6fe49d8e6329ea86822fd5e6d (MD5) Made available in DSpace on 2014-02-26T17:13:17Z (GMT). No. of bitstreams: 1 WOS000175209200002.pdf: 505294 bytes, checksum: 5d5013a6fe49d8e6329ea86822fd5e6d (MD5) Previous issue date: 2002-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:03:19Z No. of bitstreams: 1 WOS000175209200002.pdf: 505294 bytes, checksum: 5d5013a6fe49d8e6329ea86822fd5e6d (MD5) Made available in DSpace on 2014-05-20T14:03:19Z (GMT). No. of bitstreams: 1 WOS000175209200002.pdf: 505294 bytes, checksum: 5d5013a6fe49d8e6329ea86822fd5e6d (MD5) Previous issue date: 2002-04-01 Symptoms of Cucumber mosaic virus (CMV) on yellow passion flower (Passiflora edulis f. flavicarpa) are characterized by bright yellow mottling on leaves, starting at random points on the vine and diminishing in intensity towards the tip, which becomes symptomless as it grows. To determine whether symptomless portions of vines are CMV-free or represent latent infection, leaves with and without symptoms were collected from infected vines in the field. Biological, serological (plate-trapped antigen enzyme-linked immunosorbent assay, PTA-ELISA), Western blot and dot-blot hybridization assays showed that portions of the vines without symptoms were CMV-free. Vegetatively propagated vines with symptoms showed remission of symptoms on newly developed leaves. One year later, no CMV was detected in the upper leaves of these plants. Mechanically inoculated passion flower seedlings behaved similarly; symptoms were shown by few leaves after inoculation. Afterwards, plants became symptomless and CMV was not detected in the upper leaves or root system, 40 or 85 days after inoculation. The mechanism responsible for remission of symptoms accompanied by CMV disappearance is not known. USP, ESALQ, Dept Entomol Fitopatol & Zool Agr, BR-13418900 Piracicaba, SP, Brazil UNESP, IBILCE, Dept Bot, BR-15054000 Sao Jose do Rio Preto, SP, Brazil UNESP, IBILCE, Dept Bot, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
- Published
- 2002