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Column-type combined reactors were designed to cultivate micro-aerobic pentachlorophenol (PCP) degrading granular sludge under oxygen-limited conditions (0.1-0.2 mgL(-1)) over 39-day experimental period. Micro-aerobic granular had both anaerobic activity (SMA: 2.34 mMCH4/hg VSS) and aerobic activity (SOUR: 2.21 mMO2/hg VSS). Metabolite analysis results revealed that PCP was sequentially dechlorinated to TCP, DCP, and eventually to MCP. Methanogens were not directly involved in the dechlorination of PCP, but might played a vital role in stabilizing the overall structure of the granule sludge. For Eubacteria, the Shannon Index (2.09 in inoculated granular sludge) increased both in micro-aerobic granular sludge (2.61) and PCP-degradation granular sludge (2.55). However, for Archaea, it decreased from 2.53 to 1.85 and 1.84, respectively. Although the Shannon Index demonstrated slight difference between micro-aerobic granular sludge and PCP-degradation granular sludge, the Principal Component Analysis (PCA) indicated obvious variance of the microbial composition, revealing significant effect of micro-aerobic condition and PCP on microbial community. Furthermore, nucleotide sequencing indicated that the main microorganisms for PCP degradation might be related to Actinobacterium and Sphingomonas. These results provided insights into situ bioremediation of environments contaminated by PCP and had practical implications for the strategies of PCP degradation., (Copyright © 2014 Elsevier B.V. All rights reserved.)

This study systematically investigated the metabolism of pentachlorophenol (PCP) in batch experiments using coupled sludge granules under various dissolved oxygen concentrations. Results indicated that the oxygen condition in serum bottles has a significant effect on the microorganism metabolism. A greater degree of mineralization of PCP was achieved under oxygen-limited conditions (e.g., 40 and 60 initial headspace oxygen percentage (IHOP)), producing trichlorophenol (TCP), dichlorophenol (DCP) and monochlorophenol (MCP) as intermediates and chloride as one of the final products. Reductive dechlorination was identified as the primary pathway for the PCP degradation. Under strictly anaerobic or slightly oxidative conditions (0 and 20 IHOP), the reductive dechlorination of PCP led to an accumulation of TCP. Under aerobic conditions (80 and 100 IHOP), PCP degradation was less significant due to the hindered reductive chlorination in the presence of oxygen. It is also observed that cell hydrophobicity, protein (PN) concentration, settling velocity and specific gravity of the sludge granules decreased with IHOP from 0 to 60, and then increased with IHOP from 60 to 100. Specific methanogenic activity (SMA) and specific oxygen uptake rate (SOUR) confirmed that degradation of PCP was achieved by methanogenic and methanotrophic populations coexisting in a single granule. Because of a combination of reductive and oxidative degradation mechanisms, aerobic or facultative bacteria were found to oxidize the intermediates of PCP degradation products produced by methanogens and strict anaerobes during fermentation.

Coupled granules are self-immobilized aggregates of microorganisms under micro aerobic conditions, which have the dissolved oxygen (DO) level of 0.6 mg.L(-1). The effects of DO concentration on pentachlorophenol (PCP) reduction and its microbial community were investigated in a coupled anaerobic and aerobic reactor. Both the diversity and the dynamicity of the Eubacteria and Archaea community, which were responsible for PCP degradation, were evaluated by means of amplification by polymerase chain reaction (PCR) and separation using the denaturing gradient gel electrophoresis (DGGE) technique. The results demonstrated a major shift in the Eubacteria and Archaea community as the mixed aerobic and anaerobic seeding sludge (1:1 by volume) developed into coupled granules and finally acclimated with PCP throughout the experiment period within 60 days. The numbers of the Eubacteria population decreased from 20, 16 to 11; Shannon diversity index decreased from 2.75, 2.53 to 2.10. In contrast, the number of the Archaea population increased from 12, 14 to 18; and Shannon diversity index increased from 1.87, 1.88 to 2.43. Phylogenetic analysis based on 16SrDNA genes showed the dominance Sphingomonas, Desulfobulbus, Proteobacteria, Actinobacterium, Methanogenic and some uncultured bacteria in the PCP-degrading coupled granules. Microorganism community construction of coupled granules was also deduced.

Cultivation of aerobic granular sludge for pentachlorophenol (PCP) degradation under microaerobic condition (DO concentration was controlled at 0.2-0.7 mg/L) was studied in this paper. Anaerobic granules were selected as inoculum. The changes of appearance were observed and the variations of SVI, VSS/TSS, PN/PS and the size of sludge were measured during cultivating. The capabilities for degradation of PCP, AOX and COD(Cr) were also studied. Observations on mature granules were carried out by scanning electron microscope, and the results indicated bacillus was dominant on the surface of granules while in the inner of granules both bacillus and coccus were the dominant microorganisms. K, Na, Fe, Ca, Mg, Ni, Co, Mn, Cu and Zn were detected in the granules by element analysis.