1. Chicken Combs and Wattles as Sources of Bioactive Peptides: Optimization of Hydrolysis, Identification by LC-ESI-MS 2 and Bioactivity Assessment.
- Author
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Bezerra T, Estévez M, Lacerda JT, Dias M, Juliano M, Mendes MA, Morgano M, Pacheco MT, and Madruga M
- Subjects
- Animals, Antioxidants pharmacology, Benzothiazoles pharmacology, Biphenyl Compounds pharmacology, Chickens, Chromatography, Liquid methods, Collagen metabolism, Elastin metabolism, Mass Spectrometry methods, Picrates pharmacology, Protein Hydrolysates metabolism, Subtilisins metabolism, Sulfonic Acids pharmacology, Comb and Wattles drug effects, Comb and Wattles metabolism, Hydrolysis drug effects, Peptides pharmacology
- Abstract
The production of bioactive peptides from organic by-waste materials is in line with current trends devoted to guaranteeing environmental protection and a circular economy. The objectives of this study were i) to optimize the conditions for obtaining bioactive hydrolysates from chicken combs and wattles using Alcalase, ii) to identify the resulting peptides using LC-ESI-MS
2 and iii) to evaluate their chelating and antioxidant activities. The hydrolysate obtained using a ratio of enzyme to substrate of 5% ( w / w ) and 240 min of hydrolysis showed excellent Fe2+ chelating and antioxidant capacities, reducing Fe3+ and inhibiting 2, 2'-Azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The mapping of ion distribution showed that a high degree of hydrolysis led to the production of peptides with m / z ≤ 400, suggesting low mass peptides or peptides with multiple charge precursor ions. The peptides derived from the proteins of cartilage like Collagen alpha-2(I), Collagen alpha-1(I), Collagen alpha-1(III) and elastin contributed to generation of bioactive compounds. Hydrolysates from chicken waste materials could be regarded as candidates to be used as ingredients to design processed foods with functional properties.- Published
- 2020
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