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Your search keyword '"Abegg, Vanessa Fabienne"' showing total 4 results
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4 results on '"Abegg, Vanessa Fabienne"'

1. Transplacental passage of hyperforin, hypericin, and valerenic acid.

Author

Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Rath, Joshua, Treyer, Andrea, Reinehr, Michael, Kuoni, Sabrina, Oufir, Mouhssin, Potterat, Olivier, Hamburger, Matthias, and Simões-Wüst, Ana Paula

Subjects
HYPERICIN, COTYLEDONS, MENTAL illness, ACIDS, PERFUSION, HYPERICUM perforatum
Abstract

Safe medications for mild mental diseases in pregnancy are needed. Phytomedicines fromSt. John'swort and valerian are valid candidates, but safety data in pregnancy are lacking. The transplacental transport of hyperforin and hypericin (from St. John's wort), and valerenic acid (from valerian) was evaluated using the ex vivo cotyledon perfusion model (4 h perfusions, term placentae) and, in part, the in vitro Transwell assay with BeWo b30 cells. Antipyrine was used for comparison in both models. U(H)PLC-MS/MS bioanalytical methods were developed to quantify the compounds. Perfusion data obtained with term placentae showed that only minor amounts of hyperforin passed into the fetal circuit, while hypericin did not cross the placental barrier and valerenic acid equilibrated between the maternal and fetal compartments. None of the investigated compounds affected metabolic, functional, and histopathological parameters of the placenta during the perfusion experiments. Data from the Transwell model suggested that valerenic acid does not cross the placental cell layer. Taken together, our data suggest that throughout the pregnancy the potential fetal exposure to hypericin and hyperforin - but not to valerenic acid-is likely to be minimal. [ABSTRACT FROM AUTHOR]

Published
2023
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2. Placental Passage of Protopine in an Ex Vivo Human Perfusion System.

Author

Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Treyer, Andrea, Reinehr, Michael, Oufir, Mouhssin, Duong, Elisa, Potterat, Olivier, Hamburger, Matthias, and Simões-Wüst, Ana Paula

Subjects
*MATERNAL-fetal exchange, *ALKALOIDS, *PLACENTA, *PLANT extracts, *MOLECULAR structure, *PERFUSION
Abstract

The placental passage of protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. The study compound did not affect placental viability or functionality, as glucose consumption, lactate production, and beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings. [ABSTRACT FROM AUTHOR]

Published
2023
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3. Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System#.

Author

Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Treyer, Andrea, Reinehr, Michael, Oufir, Mouhssin, Duong, Elisa, Potterat, Olivier, Hamburger, Matthias, and Simões-Wüst, Ana Paula

Subjects
*BIOLOGICAL models, *CITALOPRAM, *MATERNAL-fetal exchange, *ALKALOIDS, *CELL survival, *GONADOTROPIN, *PLACENTA, *MASS spectrometry, *DESCRIPTIVE statistics, *PLANT extracts, *MOLECULAR structure, *PERFUSION, *DIAZEPAM
Abstract

The placental passage of humulone and protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Only a small portion of humulone initially present in the maternal circuit reached the fetal circuit. The humulone concentration in the maternal circuit rapidly decreased, likely due to metabolization in the placenta. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. None of the study compounds affected placental viability or functionality, as glucose consumption, lactate production, beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings. [ABSTRACT FROM AUTHOR]

Published
2021
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4. Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System#.

Author

Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Treyer, Andrea, Reinehr, Michael, Oufir, Mouhssin, Duong, Elisa, Potterat, Olivier, Hamburger, Matthias, and Simões-Wüst, Ana Paula

Subjects
BIOLOGICAL models, CITALOPRAM, MATERNAL-fetal exchange, ALKALOIDS, CELL survival, GONADOTROPIN, PLACENTA, MASS spectrometry, DESCRIPTIVE statistics, PLANT extracts, MOLECULAR structure, PERFUSION, DIAZEPAM
Abstract

The placental passage of humulone and protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Only a small portion of humulone initially present in the maternal circuit reached the fetal circuit. The humulone concentration in the maternal circuit rapidly decreased, likely due to metabolization in the placenta. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. None of the study compounds affected placental viability or functionality, as glucose consumption, lactate production, beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
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