1. Auto-fluorescent PAMAM-based dendritic molecules and their potential application in pharmaceutical sciences.
- Author
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El-Betany AMM, Kamoun EA, James C, Jangher A, Aljayyoussi G, Griffiths P, McKeown NB, and Gumbleton M
- Subjects
- Animals, Cells, Cultured, Dogs, Magnetic Resonance Spectroscopy, Dendrimers chemical synthesis, Epithelial Cells metabolism, Fluorescent Dyes chemical synthesis, Fluorescent Dyes chemistry, Permeability
- Abstract
The epithelial permeation of water-soluble fluorescent PAMAM dendrons based on 7H-benz[de] benzimidazo [2,1-a] isoquinoline-7-one as a fluorescent core across epithelial cell models MDCK I and MDCK II has been quantified. Hydrodynamic radii have been derived from self-diffusion coefficients obtained via pulsed-gradient spin-echo Nuclear Magnetic Resonance (PGSE-NMR). Results indicate that these dendritic molecules are molecularly disperse, non-aggregating, and only slightly larger than their parent homologues. MDCK I permeability studies across epithelial barriers show that these dendritic molecules are biocompatible with the chosen epithelial in-vitro model and can permeate across MDCK cell monolayers. Permeability is demonstrated to be a property of dendritic size and cell barrier restrictiveness indicating that paracellular mechanisms play the predominant role in the transport of these molecules., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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