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Your search keyword '"Elisa Guzman"' showing total 4 results
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4 results on '"Elisa Guzman"'

1. Multidrug-resistant Commensal Escherichia coli in Children, Peru and Bolivia

Author

Alessandro Bartoloni, Lucia Pallecchi, Marta Benedetti, Connie Fernandez, Yolanda Vallejos, Elisa Guzman, Ana Liz Villagran, Antonia Mantella, Chiara Lucchetti, Filippo Bartalesi, Marianne Strohmeyer, Angela Bechini, Herlan Gamboa, Hugo Rodríguez, Torkel Falkenberg, Göran Kronvall, Eduardo Gotuzzo, Franco Paradisi, and Gian Maria Rossolini

Subjects
Escherichia coli, antimicrobial resistance, commensal bacteria, resistance surveillance, Peru, Bolivia, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Using a rapid screening method, we investigated the prevalence of fecal carriage of antimicrobial drug–resistant Escherichia coli in 3,174 healthy children from 4 urban settings in Peru and Bolivia. High resistance rates were observed for ampicillin (95%), trimethoprim-sulfamethoxazole (94%), tetracycline (93%), streptomycin (82%), and chloramphenicol (70%). Lower resistance rates were observed for nalidixic acid (35%), kanamycin (28%), gentamicin (21%), and ciprofloxacin (18%); resistance to ceftriaxone and amikacin was uncommon (

Published
2006
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2. Evaluation of a rapid screening method for detection of antimicrobial resistance in the commensal microbiota of the gut

Author

Gian Maria Rossolini, Yolanda Vallejos, Franco Paradisi, Lucia Pallecchi, Torkel Falkenberg, Elisa Guzman, Mattias Larsson, Göran Kronvall, Humberto Guerra, Marianne Strohmeyer, Antonia Mantella, Ana Liz Villagran, Eduardo Gotuzzo, Alessandro Bartoloni, Marta Benedetti, Connie Fernandez, and Filippo Bartalesi

Subjects
Bolivia, food.ingredient, Direct reading, Biology, medicine.disease_cause, Sensitivity and Specificity, Microbiology, chemistry.chemical_compound, Feces, Antibiotic resistance, food, Bone plate, Drug Resistance, Bacterial, Peru, Screening method, medicine, Escherichia coli, Agar, Humans, Food science, Child, Bacteriological Techniques, Public Health, Environmental and Occupational Health, Infant, General Medicine, Antimicrobial, Infectious Diseases, chemistry, Child, Preschool, Parasitology, MacConkey agar
Abstract

The assessment of antimicrobial resistance among commensal bacteria is an indicator of the spread of antimicrobial resistance. Rapid screening methods for detection of antimicrobial-resistant faecal Escherichia coli directly on MacConkey plates have been successfully adopted but suffer from lack of standardisation. The purpose of this study was to evaluate a direct plating method (DPM) for detection of antimicrobial-resistant faecal E. coli and to compare it with a conventional method. Faecal samples were collected from 71 healthy children from Peru and Bolivia. In the DPM, a faecal swab was directly plated onto a MacConkey agar plate and antimicrobial disks were applied onto the seeded plate. Raw data were obtained by direct reading of the plate and were subjected to confirmatory analysis. Good concordance between the DPM and a conventional method was observed in detecting carriage of resistant E. coli, with a higher sensitivity for the DPM. Analysis of the results allowed interpretive criteria to be defined for DPM raw data. The DPM showed good sensitivity and specificity at very low cost (ten times cheaper than the conventional method) to investigate the faecal carriage of drug-resistant E. coli. It may represent a useful tool to conduct large-scale resistance surveillance studies and to monitor resistance control programmes cost effectively, particularly in low-resource countries.

Published
2005

3. Multidrug-resistant commensal Escherichia coli in children, Peru and Bolivia

Author

Yolanda Vallejos, Franco Paradisi, Hugo Rodriguez, Chiara Lucchetti, Marta Benedetti, Elisa Guzman, Gian Maria Rossolini, Herlan Gamboa, Connie Fernandez, Filippo Bartalesi, Antonia Mantella, Göran Kronvall, Ana Liz Villagran, Lucia Pallecchi, Torkel Falkenberg, Alessandro Bartoloni, Eduardo Gotuzzo, Marianne Strohmeyer, and Angela Bechini

Subjects
Microbiology (medical), DNA, Bacterial, Male, Bolivia, Nalidixic acid, Urban Population, Epidemiology, Tetracycline, lcsh:Medicine, Microbial Sensitivity Tests, Biology, Polymerase Chain Reaction, lcsh:Infectious and parasitic diseases, Microbiology, Cohort Studies, Feces, Antibiotic resistance, fluids and secretions, Ampicillin, Drug Resistance, Multiple, Bacterial, Peru, medicine, Escherichia coli, Prevalence, Humans, lcsh:RC109-216, antimicrobial resistance, Child, commensal bacteria, Research, lcsh:R, Infant, biochemical phenomena, metabolism, and nutrition, Virology, Multiple drug resistance, Ciprofloxacin, Infectious Diseases, Amikacin, Streptomycin, resistance surveillance, Child, Preschool, Female, medicine.drug
Abstract

Healthy children in urban areas have a high prevalence of fecal carriage of drug-resistant E. coli., Using a rapid screening method, we investigated the prevalence of fecal carriage of antimicrobial drug–resistant Escherichia coli in 3,174 healthy children from 4 urban settings in Peru and Bolivia. High resistance rates were observed for ampicillin (95%), trimethoprim-sulfamethoxazole (94%), tetracycline (93%), streptomycin (82%), and chloramphenicol (70%). Lower resistance rates were observed for nalidixic acid (35%), kanamycin (28%), gentamicin (21%), and ciprofloxacin (18%); resistance to ceftriaxone and amikacin was uncommon (

4. Uso de pruebas rápidas inmunocromatográficas para la detección de Plasmodium falciparum en donantes de sangre en Perú

Author

Nancy Arróspide V, Maritza Puray C, Elisa Guzmán S, Milton Verano B, Sigifredo Medina R, Luz Mendizábal A, and Sonia Gonzáles G

Subjects
Donadores de sangre, Plasmodium falciparum, Malaria, Técnicas y Procedimientos Diagnósticos, Peru, Medicine, Medicine (General), R5-920
Abstract

Objetivo: Evaluar el grado de utilidad de la prueba rápida OptiMal DiaMed ® para diagnóstico de malaria por Plasmodium falciparum en donantes de sangre en lugares con alto mediano y bajo riesgo de transmisión de malaria en Perú. Material y Métodos: Estudio prospectivo, multicéntrico, realizado en 8 bancos de sangre de 4 regiones del país. Se evaluó la especificidad, sensibilidad, valor predictivo positivo y valor predictivo negativo de la prueba OptiMal DiaMed ® en un total de 918 donantes de sangre frente a la gota gruesa como prueba patrón de oro. Resultados: Se detectó un caso positivo para ambas pruebas, 16 falsos positivos y ningún falso negativo. El OptiMal Diamed ®, en este estudio, tuvo una sensibilidad de 100% y una especificidad de 98%, con un valor predictivo positivo de 5,5% y un valor predictivo negativo de 100%. Conclusiones: el OptiMal DiaMed ® tiene buenos niveles de especificidad y sensibilidad, y puede ser utilizada como prueba de tamizaje para diagnóstico de malaria por Plasmodium falciparum en bancos de sangre.

Published
2004

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