1. Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity.
- Author
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Mashiba R, Ishikawa M, Sumiya YU, Kawakatsu K, Tran NK, and Nishikata T
- Subjects
- Cell Line, Humans, Macrophage Activation physiology, Macrophage Activation drug effects, Macrophage Activation immunology, Macrophage-Activating Factors pharmacology, Macrophages drug effects, Macrophages immunology, Phagocytosis physiology
- Abstract
Background/aim: Serum-derived macrophage activating factor (serum MAF) is known to increase the phagocytic activity of macrophages and potentially plays a role in activating cancer immunity. In order to reveal the contributing factors for phagocytic activation, the migratory activity and the efficiency of engulfment was analyzed., Materials and Methods: THP-1 macrophages were induced by 12-O-tetradecanoyl-13-acetate (TPA). The migratory activity and efficiency of engulfment were analyzed by time-lapse imaging and suspension assay, respectively., Results: While the distance of migration did not change before and after activation with serum MAF, the efficiency of beads internalisation was significantly increased., Conclusion: Phagocytic activation of serum-MAF-treated macrophages was caused by increasing the efficiency of engulfment. This study contributes to the knowledge about the activation of the immune system through phagocytic activation of macrophages., (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2018
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