1. Application of Paper Strip Extraction in Combination with LC-MS-MS in Pharmacokinetics.
- Author
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Legette, LeeCole L., Reed, Ralph L., Murty, Lia, Maier, Claudia S., and Stevens, Jan F.
- Subjects
EXTRACTION (Chemistry) ,PHARMACEUTICAL industry ,LIQUID chromatography-mass spectrometry ,PHARMACOKINETICS ,DRIED blood spot testing ,LIQUID-liquid extraction ,BUTYL methyl ether - Abstract
Recent advances in sampling techniques in the pharmaceutical industry sparked significant interest in applying improvements to extraction methods for greater analyte detection and quantitation. In particular, the dried blood spot (DBS) sampling technique has numerous advantages compared to traditional methods such as liquid-liquid extraction, including the use of small sample volumes, less sample processing, and less exposure to toxic solvents (ether, methyl tertbutyl ether [MTBE], and dichloromethane). In this article, we discuss the adaptation of DBS technology to develop and validate a novel paper strip extraction method for the analysis of natural product metabolites in biological samples obtained from a human pharmacokinetic study of xanthohumol, a hop prenylflavonoid. [ABSTRACT FROM AUTHOR]
- Published
- 2014