1. Adenosine monophosphate boosts the cryoprotection of ultrasound-assisted freezing to frozen surimi: Insights into protein structures and gelling behaviors.
- Author
-
Zhu, Xiangwei, He, Diheng, Chen, Yingying, Duan, Xinyu, Li, Yonghui, Yuan, Yue, Zhan, Fuchao, Li, Bin, and Teng, Yongxin
- Subjects
- *
ADENOSINE monophosphate , *PROTEIN structure , *SURIMI , *FROZEN foods industry , *DENATURATION of proteins , *SORBITOL , *PHOSPHATES , *POLYOLS - Abstract
Ultrasound-assisted freezing (UAF) is a clean technique for meat cryoprotections; however, its effectiveness is still limited compared to conventional cryoprotectants, e.g., sugars, polyols, especially at high dosages. To resolve this problem, a synergistic cryoprotection strategy was developed in this study. Adenosine monophosphate (AMP), an adenosine-type food additive, was introduced into frozen surimi at a considerably reduced content (0.08%), yet substantially enhanced the efficiency of UAF to comparable levels of commercial cryoprotectant (4% sucrose with 4% sorbitol). Specifically, UAF/AMP treatment retarded denaturation of surimi myofibrillar protein (MP) during 60-day frozen storage, as evidenced by its increased solubility, Ca2+-ATPase activity, sulfhydryl content, declined surface hydrophobicity, particle size, and stabilized protein conformation. Gels of UAF/AMP-treated surimi also demonstrated more stabilized microstructures, uniform water distributions, enhanced mechanical properties and water-holding capacities. This study provided a feasible approach to boost the cryoprotective performance of UAF, thus expanding its potential applications in frozen food industry. [Display omitted] • Cryoprotections of ultrasound-assisted freezing (UAF) were improved to frozen surimi. • Only 0.1% adenosine phosphate (AMP) was used during UAF processing. • UAF/AMP treatment inhibited the denaturation of myofibrillar protein. • Effectiveness of UAF/AMP was comparable to the commercial cryoprotectant. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF