1. PHO2-dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis.
- Author
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Liu TY, Huang TK, Tseng CY, Lai YS, Lin SI, Lin WY, Chen JW, and Chiou TJ
- Subjects
- Arabidopsis genetics, Arabidopsis Proteins genetics, Gene Expression Regulation, Plant genetics, Gene Expression Regulation, Plant physiology, Homeostasis genetics, Homeostasis physiology, Mutation, Missense genetics, Plant Roots genetics, Plant Roots metabolism, Plant Shoots genetics, Plant Shoots metabolism, Plants, Genetically Modified genetics, Ubiquitin-Conjugating Enzymes genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Phosphates metabolism, Plants, Genetically Modified metabolism, Ubiquitin-Conjugating Enzymes metabolism
- Abstract
The Arabidopsis thaliana pho2 mutant, which is defective in a ubiquitin-conjugating E2 enzyme, displays inorganic phosphate (Pi) toxicity as a result of enhanced uptake and root-to-shoot translocation of Pi. To elucidate downstream components of the PHO2-dependent regulatory pathway, we identified two pho2 suppressors as carrying missense mutations in PHO1, which has been implicated in Pi loading to the xylem. In support of the genetic interaction between PHO1 and PHO2, we found that the protein level of PHO1 is increased in pho2, whereas such accumulation is ameliorated in both pho2 suppressors. Results from cycloheximide and endosomal Cys protease inhibitor E-64d treatments further suggest that PHO1 degradation is PHO2 dependent and involves multivesicular body-mediated vacuolar proteolysis. Using the transient expression system of tobacco (Nicotiana tabacum) leaves, we demonstrated that PHO1 and PHO2 are partially colocalized and physically interact in the endomembranes, where the ubiquitin conjugase activity of PHO2 is required for PHO1 degradation. In addition, reduced PHO1 expression caused by PHO1 mutations impede Pi uptake, indicating a functional association between xylem loading and acquisition of Pi. Together, our findings uncover a pivotal molecular mechanism by which PHO2 modulates the degradation of PHO1 in the endomembranes to maintain Pi homeostasis in plants.
- Published
- 2012
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