1. Raf kinase inhibitor protein expression in smooth muscle tumours of the uterus: a diagnostic marker for leiomyosarcoma?
- Author
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Greco S, Pinheiro J, Cardoso-Carneiro D, Giantomassi F, Pellegrino P, Scaglione G, Delli Carpini G, Ciavattini A, Zannoni GF, Goteri G, Martinho O, and Ciarmela P
- Subjects
- Humans, Female, Smooth Muscle Tumor metabolism, Smooth Muscle Tumor pathology, Smooth Muscle Tumor diagnosis, Cell Line, Tumor, Middle Aged, Cell Movement, Adult, Immunohistochemistry, Leiomyosarcoma metabolism, Leiomyosarcoma pathology, Leiomyosarcoma diagnosis, Uterine Neoplasms metabolism, Uterine Neoplasms pathology, Uterine Neoplasms genetics, Phosphatidylethanolamine Binding Protein metabolism, Phosphatidylethanolamine Binding Protein genetics, Leiomyoma metabolism, Leiomyoma pathology, Leiomyoma diagnosis, Biomarkers, Tumor metabolism
- Abstract
Research Question: What is the expression pattern of Raf kinase inhibitory protein (RKIP) in different subtypes of leiomyoma (usual type, cellular, apoplectic or haemorrhagic leiomyoma, leiomyoma with bizarre nuclei and lipoleiomyoma) and leiomyosarcoma specimens, and what is its biological role in leiomyosarcoma cells?, Design: Leiomyoma and leiomyosarcoma specimens underwent immunohistochemistry staining. Leiomyosarcoma SK-LMS-1 cell line was RKIP knocked down and RKIP overexpressed, and cell viability, wound healing migration and clonogenicity assays were carried out., Results: A higher immunohistochemical expression of RKIP was observed in bizarre leiomyomas, than in usual-type leiomyomas. Decreased expression was also found in cellular leiomyoma, with generally absent staining in leiomyosarcomas. Upon RKIP expression manipulation in SK-LMS-1 cell line, no major differences were observed in cell viability and migration capacity over time. RKIP knockout, however, resulted in a significant increase in the cell's ability to form colonies (P = 0.011)., Conclusion: RKIP distinct expression pattern among leiomyoma histotype and leiomyosarcoma, and its effect on leiomyosarcoma cells on colony formation, encourages further studies of RKIP in uterine smooth muscle disorders., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2024
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