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Your search keyword '"Western blotting analysis"' showing total 2 results
Start Over Descriptor "Western blotting analysis" Topic phospholipids
2 results on '"Western blotting analysis"'

1. Use of magnoflorine-phospholipid complex to permeate blood-brain barrier and treat depression in the CUMS animal model.

Author

Li B, Han L, Cao B, Yang X, Zhu X, Yang B, Zhao H, and Qiao W

Subjects
Animals, Antidepressive Agents administration & dosage, Antidepressive Agents blood, Aporphines administration & dosage, Aporphines blood, Behavior, Animal drug effects, Depression metabolism, Disease Models, Animal, Male, Mice, Inbred ICR, Permeability, Antidepressive Agents therapeutic use, Aporphines therapeutic use, Blood-Brain Barrier metabolism, Depression drug therapy, Drug Carriers chemistry, Phospholipids chemistry
Abstract

To improve the liposolubility and blood-brain barrier permeability of magnoflorine, a new formulation of magnoflorine-phospholipid complex was prepared, characterized, and pharmacologically evaluated in the chronic unpredictable mild stress animal model. In this paper, the magnoflorine-phospholipid complex was synthesized and its characterization was determined. The antidepressant-like and antioxidant activity of magnoflorine-phospholipid complex was investigated by behavioral tests and western blotting analysis. As a result, the magnoflorine-phospholipid complex displayed high encapsulation efficiency and significantly improved the oil-water participate coefficient. In vivo blood-brain distribution study, the magnoflorine-phospholipid complex extended the duration of magnoflorine in blood and help magnoflorine to permeate the blood-brain barrier into brain. In behavioral tests, the magnoflorine-phospholipid complex significantly decreased immobility time compared to model control group in both FST and TST. Furthermore, the magnoflorine-phospholipid complex increased the expression of antioxidative stress-related proteins by the western blotting analysis. These findings strongly suggest that the phospholipid complex could significantly improve liposolubility, drug properties of magnoflorine and help magnoflorine permeate blood-brain barrier and exert the antidepressant effect.

Published
2019
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2. Use of magnoflorine-phospholipid complex to permeate blood-brain barrier and treat depression in the CUMS animal model

Author

Xiaoying Yang, Bingjie Li, Qiao Wei, Linmeng Han, Xuehui Zhu, Haodong Zhao, Bingyan Cao, and Bing Yang

Subjects
Male, Drug, Aporphines, Antioxidant, media_common.quotation_subject, medicine.medical_treatment, western blotting analysis, Pharmaceutical Science, Pharmacology, Blood–brain barrier, Permeability, chemistry.chemical_compound, physicochemical characterization, In vivo, medicine, Animals, pharmacological evaluation, Phospholipids, media_common, Drug Carriers, Mice, Inbred ICR, Behavior, Animal, Depression, lcsh:RM1-950, General Medicine, in vivo blood-brain distribution, Permeation, Antidepressive Agents, Blot, Disease Models, Animal, lcsh:Therapeutics. Pharmacology, Magnoflorine-phospholipid complex, medicine.anatomical_structure, chemistry, Blood-Brain Barrier, Antidepressant, lipids (amino acids, peptides, and proteins), Magnoflorine, Research Article
Abstract

To improve the liposolubility and blood-brain barrier permeability of magnoflorine, a new formulation of magnoflorine-phospholipid complex was prepared, characterized, and pharmacologically evaluated in the chronic unpredictable mild stress animal model. In this paper, the magnoflorine-phospholipid complex was synthesized and its characterization was determined. The antidepressant-like and antioxidant activity of magnoflorine-phospholipid complex was investigated by behavioral tests and western blotting analysis. As a result, the magnoflorine-phospholipid complex displayed high encapsulation efficiency and significantly improved the oil-water participate coefficient. In vivo blood-brain distribution study, the magnoflorine-phospholipid complex extended the duration of magnoflorine in blood and help magnoflorine to permeate the blood-brain barrier into brain. In behavioral tests, the magnoflorine-phospholipid complex significantly decreased immobility time compared to model control group in both FST and TST. Furthermore, the magnoflorine-phospholipid complex increased the expression of antioxidative stress-related proteins by the western blotting analysis. These findings strongly suggest that the phospholipid complex could significantly improve liposolubility, drug properties of magnoflorine and help magnoflorine permeate blood-brain barrier and exert the antidepressant effect.

Published
2019
Full Text
View/download PDF

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