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Differences in root morphology and acclimation to low-phosphorus (P) soil were examined among eight legume species from the Trifolium Section Tricocephalum to understand how these root attributes determine P acquisition. Ornithopus sativus was included as a highly P-efficient benchmark species. Plants were grown as microswards in pots with five rates of P supplied in a topsoil layer to mimic uneven P distribution within a field soil profile. Topsoil and subsoil roots were harvested separately to enable measurement of the nutrient-foraging responses. Critical P requirement (lowest P supply for maximum yield) varied over a threefold range, reflecting differences in root morphology and acclimation of nutrient-foraging roots to P stress. Among the species, there was a 3.2-fold range in root length density, a 1.7-fold range in specific root length, and a 2.1-fold range in root hair length. O. sativus had the lowest critical P requirement, displayed a high root length density, the highest specific root length, and the longest root hairs. Acquisition of P from P-deficient soil was facilitated by development of a large root hair cylinder (i.e. a large root-soil interface). This, in turn, was determined by the intrinsic root morphology attributes of each genotype, and the plasticity of its root morphology response to internal P stress. Root acclimation in low-P soil by all species was mostly associated with preferential allocation of mass to nutrient-foraging roots. Only O. sativus and four of the Trifolium species adjusted specific root length beneficially, and only O. sativus increased its root hair length in low-P soil., (© 2021 Scandinavian Plant Physiology Society.)

The mainstream pasture legume species such as Trifolium subterraneum, T. repens and annual Medicago spp. used in the temperate pasture systems of southern Australia have high critical external requirements for phosphorus (P) (i.e. P required to achieve 90% of maximum yield). This work aimed to identify alternative pasture legume species that could be used in systems with lower P input. Shoot and root biomass of 12 species of pasture legume was measured in response to seven rates of P applied to the top 48 mm of soil in a pot experiment. Most species had maximum yields similar to T. subterraneum, but some required only one-third of the applied P to achieve this. The critical external P requirement of the species, ranked from lowest to highest, was as follows: Ornithopus compressus = O. sativus < Biserrula pelecinus < T. michelianum = T. vesiculosum = T. glanduliferum < T. hirtum = Medicago truncatula = T. purpureum = T. incarnatum < T. spumosum = T. subterraneum. An ability to maximise soil exploration through a combination of high root-length density, high specific root length and long root hairs (i.e. a large specific root-hair-cylinder volume) was associated with a low critical external P requirement. The results indicate that Ornithopus spp. could be used to achieve productive, low P-input pasture systems. [ABSTRACT FROM AUTHOR]