5 results on '"Ilyas, Sobia"'
Search Results
2. Termitomyces pakistanensis, a new mushroom species from Pakistan based on scanning electron microscopy and ITS‐rDNA barcoding.
- Author
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Razaq, Abdul, Ishaq, Aamna, Ilyas, Sobia, Niaz, Shereen, and Sadia, Sehrish
- Abstract
Termitomyces pakistanensis sp. nov. is a member of an edible genus generally distributed in Asia and Europe. This species has been described as new species based on its different morphology, and scanning electron microscopy (SEM) of basidiospores. The novelty and degree of endemism is confirmed by analyzing the genetic variation of the internal transcribed spacer regions (ITS1–5.8 S–ITS2) of the ribosomal DNA gene, a universal fungal marker. The evolutionary affinities of new species is also evaluated with Asian and European species by phylogenetic analysis based on ITS sequences. In our phylogenetic analysis, this genus is found monophyletic comprising of two monophyletic sub clades: Clade I, Microcarpus, with small sized fruiting bodies generally less than 5 cm without pseudorrhiza and Clade II, Macrocarpus, with large sized fruiting bodies generally more than 5 cm having pseudorrhiza. All collections of Pakistani species clustered independently in Microcarpus clade showing their endemic genetic makeup as it is clustering independently. A comprehensive description, photographs of the basidiocarps and Scanning electron microscopy (SEM) micrographs of spores are provided. Research Highlights: It has a new species from Pakistan to world based on the scanning electron microscopy and further confirmed by DNA barcoding.The exact shape and size of basidiospores of this novel species is first time introduced by using SEM analysis.This genus is rarely described from Pakistan.This paper has introduced a two clade, Microcarpus and macrocarpus, in the world for this genus. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Fungal Systematics and Evolution: FUSE 4
- Author
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Liu, Li-Na, Razaq, Abdul, Atri, Narender Singh, Bau, Tolgor, Belbahri, Lassaad, Bouket, Ali Chenari, Chen, Lai-Ping, Deng, Chu, Ilyas, Sobia, Khalid, Abdul Nasir, Kitaura, Marcos Junji, Kobayashi, Takahito, Li, Yu, Lorenz, Aline Pedroso, Ma, Yuan-Hao, Malysheva, Ekaterina, Malysheva, Vera, Nuytinck, Jorinde, Qiao, Min, Saini, Munruchi Kaur, Scur, Mayara Camila, Sharma, Samidha, Shu, Li-Li, Spirin, Viacheslav, Tanaka, Yoshikazu, Tojo, Motoaki, Uzuhashi, Shihomi, Valerio-Junior, Claudio, Verbeken, Annemieke, Verma, Balwant, Wu, Ri-Han, Xu, Jian-Ping, Yu, Ze-Fen, Zeng, Hui, Zhang, Bo, Banerjee, Arghya, Beddiar, Arifa, Bordallo, Juan-Julian, Dafri, Ahlem, Dima, Balint, Krisai-Greilhuber, Irmgard, Lorenzini, Marilinda, Mandal, Raghunath, Morte, Asuncion, Nath, Partha Sarathi, Papp, Viktor, Pavlik, Jozef, Rodriguez, Antonio, Sevcikova, Hana, Urban, Alexander, Voglmayr, Hermann, Zapparoli, Giacomo, Finnish Museum of Natural History, and Botany
- Subjects
1 validation ,Didymosphaeriaceae ,MOLECULAR PHYLOGENY ,ectomycorrhizal fungi ,aquatic hyphomycetes ,Ambicystidiati ,Marasmiaceae ,cyanolichens ,phylogeny ,Pluteaceae ,MULTIPLE SEQUENCE ALIGNMENT ,Herpotrichiellaceae Inocybaceae ,taxonomy ,PHYLOGENETIC-RELATIONSHIPS ,1 new combination ,Carpaticum ,morphology ,Agariconycetes, Ambicystidiati, Bolbitiaceae, Boletales, Didymosphaeriaceae, Herpotrichiellaceae Inocybaceae, Marasmiaceae, Pezizaceae, Pluteaceae, aquatic hyphomycetes, barcoding, Carpaticum, cyanolichens, desert truffle, ectomycorrhizal fungi, ITS ,morphology, phylogeny, taxonomy, type studies, 8 new species, 1 new combination, 1 validation ,Pezizaceae ,MAXIMUM-LIKELIHOOD ,GENUS INOCYBE ,Bolbitiaceae ,Agariconycetes ,type studies ,DESERT TRUFFLES ,CRINIPELLIS BASIDIOMYCOTA ,desert truffle ,barcoding ,INTERNAL TRANSCRIBED SPACER ,8 new species ,Agarico?nycetes ,SUBUNIT RIBOSOMAL DNA ,1181 Ecology, evolutionary biology ,SECTION RIMOSAE ,Boletales ,ITS - Abstract
Eight new species presented are Calostoma areolatum collected in Wuyishan National Park (China), Crinipellis bidens from Hubei Province (China), Lactifluus sainii from Himalayan India, Inocybe elata from Yunnan (China), Inocybe himalayensis from Pakistan. Specimens previously identified as Massalongia carnosa represent a new species, namely M. patagonica restricted to southern South America. Saprolegnia maragheica is a new oomycete species of fresh water in Maraghe (Iran). Uncispora wuzhishanensis is a new aquatic hyphomycete species. A type specimen of Raddetes turkestanicus was studied and based on this the new combination Conocybe turkestanica, is proposed. Argyranthemum frutescens is a new host for Alternaria alternata and Syzygium cumini for Phyllosticta capitalensis in India. Crepidotus ehrendorferi is confirmed for Hungary and Pluteus leucoborealis for Central Europe, and for the phytogeographical region of Carpaticum. Pseudopithomyces palmicola is shown to occur on grapevine and it is validated by adding a unique identifier. Terfezia fanfani is reported first from Algeria.
- Published
- 2018
4. Phylogeny and Taxonomy of Hebeloma theobrominum and H. mesophaeum from Western Himalaya.
- Author
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Razaq, Abdul, Ilyas, Sobia, and Khalid, Abdul Nasir
- Subjects
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PHYLOGENY , *HEBELOMA , *RECOMBINANT DNA , *GENETIC barcoding , *PHENOTYPES - Abstract
This study deals with the molecular systematics of Asian collections of Hebeloma theobrominum and H. mesophaeum which have not been previously reported from any Himalayan part of the world, and are new to Pakistan. Both were identified using morpho-anatomical and molecular characterization. The target rDNA of both species was amplified by using universal fungal primers. In phylogenetic analysis, both species distinctly clustered with their respective groups. These both species have been described first time from Asia using ITS-rDNA barcoding. The sequences of both species were compared with those of same European species from the GenBank. These species clustered with European species in phylogenetic tree proving their cosmopolitan distribution. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
5. Molecular Identification of Lepiota acutesquamosa and L. cristata (Basidiomycota, Agaricales) Based on ITS-rDNA Barcoding from Himalayan Moist Temperate Forests of Pakistan.
- Author
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Razaq, Abdul, Khalid, Abdul Nasir, and Ilyas, Sobia
- Subjects
LEPIOTA ,RIBOSOMAL DNA ,POLYMERASE chain reaction ,NUCLEOTIDE sequence ,DNA primers ,FUNGAL phylogeny ,FUNGAL morphology - Abstract
Lepiota acutesquamosa and L. cristata (Basidiomycota, Agaricales) collected from Himalayan moist temperate forests of Pakistan were characterized using internal transcribed spacers (ITS) of rNDA, a fungal molecular marker. The ITS-rDNA of both species was analyzed using polymerase chain reaction (PCR) and DNA sequencing. The target region when amplified using universal fungal primers (ITS1F and ITS4) generated 650-650bp fragments. Consensus sequences of both species were submitted for initial blast analysis which revealed and confirmed the identification of both species by comparing the sequences of these respective species already present in the GenBank. Sequence of Pakistani collection of L. acutesquamosa matched 99% with sequences of same species (FJ998400) and Pakistani L. cristata matched 97% with its sequences (EU081956, U85327, AJ237628). Further, in phylogenetic analysis both species distinctly clustered with their respective groups. Morphological characters like shape, size and color of basidiomata, basidiospore size, basidial lengths, shape and size of cheilocystidia of both collections were measured and compared. Both these species have been described first time from Pakistan on morph-anatomical and molecular basis. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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