1. Serratia inhibens sp. nov., a new antifungal species isolated from potato ( Solanum tuberosum ).
- Author
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Hennessy RC, Dichmann SI, Martens HJ, Zervas A, and Stougaard P
- Subjects
- Bacterial Typing Techniques, Base Composition, DNA, Bacterial genetics, Denmark, Fatty Acids chemistry, Genes, Bacterial, Nucleic Acid Hybridization, Phospholipids chemistry, RNA, Ribosomal, 16S genetics, Rhizosphere, Sequence Analysis, DNA, Serratia isolation & purification, Ubiquinone chemistry, Vitamin K 2 analogs & derivatives, Vitamin K 2 chemistry, Antibiosis, Phylogeny, Serratia classification, Solanum tuberosum microbiology
- Abstract
A novel bacterial strain, S40
T , with strong antifungal activity was isolated from the rhizosphere of green potato collected from Zealand, Denmark. Polyphasic analysis with a combined phenotypic, phylogenetic and genomic approach was used to characterize S40T . Phylogenetic analysis based on the 16S rRNA gene and MLSA (concatenated gyrB , rpoD , infB and atpD sequences) showed that strain S40T was affiliated with the genus Serratia and with Serratia plymuthica PRI-2C as the closest related strain [average nucleotide identity (ANI), 99.26 %; DNA-DNA hybridization (dDDH), 99.20%]. However, whole genome sequence analyses revealed that S40T and S. plymuthica PRI-2C genomes displayed lower similarities when compared to all other S. plymuthica strains (ANI ≤94.34 %; dDDH ≤57.6 % relatedness). The DNA G+C content of strain S40T was determined to be 55.9 mol%. Cells of the strain were Gram-negative, rod-shaped, facultative anaerobic and displayed growth at 10-37 °C (optimum, 25-30 °C) and at pH 6-9 (optimum, pH 6-7). Major fatty acids were C16 : 0 (27.9 %), summed feature (C16 : 1 ω 6 c /C16 : 1 ω7 c ; 18.0 %) and C17 : 0 cyclo (15.1 %). The respiratory quinone was determined to be Q8 (94 %) and MK8 (95 %) and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The results of phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S40T represents a novel species of the genus Serratia , for which the name Serratia inhibens sp. nov. is proposed. The type strain is S40T (=LMG 31467T =NCIMB 15235T ). In addition, we propose that S. plymuthica PRI-2C is reclassified and transferred to the species S. inhibens as S. inhibens PRI-2C.- Published
- 2020
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