1. Fluorescent complex of pyoverdin with aluminum.
- Author
-
del Olmo A, Caramelo C, and SanJose C
- Subjects
- Ferric Compounds chemistry, Fluorescence, Pseudomonas fluorescens chemistry, Siderophores chemistry, Aluminum chemistry, Oligopeptides, Pigments, Biological chemistry
- Abstract
When a pyoverdin (PV), (a siderophore) from Pseudomonas fluorescens, binds aluminum 1:1, its natural fluorescence almost doubles, whereas PV-Fe is non-fluorescent. Complex formation allows [Al] determination down to 1 mug/l. Fe(III) in the sample interferes with [Al] determination, but added after PV, improves the assay's performance. Ascorbic acid does not eliminate Fe(III) interference. PV-Al fluorescence could have analytical and toxicological applications.
- Published
- 2003
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