1. Evidence for contamination as the origin for bacteria found in human placenta rather than a microbiota
- Author
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Sandra Wydau-Dematteis, Sean Kennedy, Rémi Gschwind, Vassilis Tsatsaris, Anne-Gaël Cordier, Marie-José Butel, Frédéric Barbut, Thierry Fournier, Physiopathologie et pharmacotoxicologie placentaire humaine : Microbiote pré & post natal (3PHM - UMR-S 1139), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), PremUp Foundation, Institut de Recherche pour le Développement (IRD)-CHI Créteil-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris-Saclay-Université Paris Cité (UPCité), Département de Biologie Computationnelle - Department of Computational Biology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by a PRIDE grant from the hospital-university department 'Risks in Pregnancy' and by Paris Descartes university., and Kennedy, Sean
- Subjects
0301 basic medicine ,Embryology ,Placenta ,Extraembryonic Membranes ,Physiology ,Umbilical cord ,Umbilical Cord ,Database and Informatics Methods ,0302 clinical medicine ,MESH: Pregnancy ,Pregnancy ,Medicine and Health Sciences ,MESH: Chorionic Villi ,DNA extraction ,reproductive and urinary physiology ,030219 obstetrics & reproductive medicine ,Multidisciplinary ,medicine.diagnostic_test ,Microbiota ,Genomics ,MESH: Cesarean Section ,MESH: Placenta ,MESH: Chorionic Villi Sampling ,medicine.anatomical_structure ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Chorionic Villi Sampling ,Obstetric Procedures ,Medical Microbiology ,In utero ,embryonic structures ,Chorionic villi ,Medicine ,Female ,Chorionic Villi ,Sequence Analysis ,Research Article ,Adult ,DNA, Bacterial ,Bioinformatics ,Science ,Sequence Databases ,Chorionic villus sampling ,Surgical and Invasive Medical Procedures ,Microbial Genomics ,Biology ,Microbiology ,Specimen Handling ,03 medical and health sciences ,Extraction techniques ,Genetics ,medicine ,Humans ,MESH: Microbiota ,Microbiome ,MESH: Specimen Handling ,[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology ,Fetus ,MESH: Humans ,Bacteria ,MESH: Umbilical Cord ,Cesarean Section ,MESH: Extraembryonic Membranes ,Gut Bacteria ,Organisms ,Biology and Life Sciences ,MESH: Adult ,Delivery, Obstetric ,medicine.disease ,MESH: DNA, Bacterial ,Research and analysis methods ,MESH: Bacteria ,Biological Databases ,030104 developmental biology ,MESH: Delivery, Obstetric ,Metagenomics ,MESH: Female ,Developmental Biology - Abstract
International audience; Until recently the in utero environment of pregnant women was considered sterile. Recent high-sensitivity molecular techniques and high-throughput sequencing lead to some evidence for a low-biomass microbiome associated with the healthy placenta. Other studies failed to reveal evidence for a consistent presence of bacteria using either culture or molecular based techniques. Comparing conflicting "placental microbiome" studies is complicated by the use of varied and inconsistent protocols. Given this situation, we undertook an evaluation of the in utero environment sterility using several controlled methods, in the same study, to evaluate the presence or absence of bacteria and to explain contradictions present in the literature. Healthy pregnant women (n = 38) were recruited in three maternity wards. Placenta were collected after cesarean section with or without Alexis ® and vaginal delivery births. For this study we sampled fetal membranes, umbilical cord and chorionic villi. Bacterial presence was analyzed using bacterial culture and qPCR on 34 fetal membranes, umbilical cord and chorionic villi samples. Shotgun metagenomics was performed on seven chorionic villi samples. We showed that the isolation of meaningful quantities of viable bacteria or bacterial DNA was possible only outside the placenta (fetal membranes and umbilical cords) highlighting the importance of sampling methods in studying the in utero environment. Bacterial communities described by metagenomics analysis were similar in chorionic villi samples and in negative controls and were dependent on the database chosen for the analysis. We conclude that the placenta does not harbor a specific, consistent and functional microbiota.
- Published
- 2020