1. Immune landscape of human placental villi using single-cell analysis.
- Author
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Toothaker JM, Olaloye O, McCourt BT, McCourt CC, Silva TN, Case RM, Liu P, Yimlamai D, Tseng G, and Konnikova L
- Subjects
- Antigens, CD metabolism, Antigens, Differentiation, Myelomonocytic metabolism, B-Lymphocytes cytology, B-Lymphocytes immunology, B-Lymphocytes metabolism, B7-H1 Antigen genetics, B7-H1 Antigen metabolism, Chorionic Villi metabolism, Female, Fetus immunology, Fetus metabolism, Flow Cytometry, HLA-DR Antigens genetics, HLA-DR Antigens metabolism, Humans, Killer Cells, Natural cytology, Killer Cells, Natural immunology, Killer Cells, Natural metabolism, Leukocyte Common Antigens metabolism, Lymphocyte Activation, Macrophages cytology, Macrophages immunology, Macrophages metabolism, Memory T Cells cytology, Memory T Cells immunology, Memory T Cells metabolism, Placenta cytology, Placenta metabolism, Pregnancy, Pregnancy Trimester, Second, Receptors, Cell Surface metabolism, Receptors, Chemokine genetics, Receptors, Chemokine metabolism, Single-Cell Analysis methods, T-Lymphocytes cytology, T-Lymphocytes immunology, T-Lymphocytes metabolism, Chorionic Villi immunology, Placenta immunology
- Abstract
Maintenance of a healthy pregnancy is reliant on a successful balance between the fetal and maternal immune systems. Although the maternal mechanisms responsible have been well studied, those used by the fetal immune system remain poorly understood. Using suspension mass cytometry and various imaging modalities, we report a complex immune system within the mid-gestation (17-23 weeks) human placental villi (PV). Consistent with recent reports in other fetal organs, T cells with memory phenotypes, although rare in abundance, were detected within the PV tissue and vasculature. Moreover, we determined that T cells isolated from PV samples may be more proliferative after T cell receptor stimulation than adult T cells at baseline. Collectively, we identified multiple subtypes of fetal immune cells within the PV and specifically highlight the enhanced proliferative capacity of fetal PV T cells., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2022. Published by The Company of Biologists Ltd.)
- Published
- 2022
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