1. The Prl3d1-Cre mouse line selectively induces the expression of Cre recombinase in parietal trophoblast giant cells.
- Author
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Pan L, Zhu F, Yu A, Jiang Y, Wang D, Zhou M, Jia C, Cui Y, Tang L, Tang H, and Li J
- Subjects
- Animals, Female, Mice, Pregnancy, Giant Cells metabolism, Integrases genetics, Integrases metabolism, Mice, Transgenic, Placenta metabolism, Red Fluorescent Protein, Trophoblasts
- Abstract
The placenta plays a pivotal role in the maintenance of normal pregnancy, but how it forms, matures, and performs its function remains poorly understood. Here, we describe a novel mouse line (Prl3d1-iCre) that expresses iCre recombinase under the control of the endogenous prl3d1 promoter. Prl3d1 has been proposed as a marker for distinguishing trophoblast giant cells (TGCs) from other trophoblast cells in the placenta. The in vivo efficiency and specificity of the Cre line were analyzed by interbreeding Prl3d1-iCre mice with B6-G/R reporter mice. Through anatomical studies of the placenta and other tissues of Prl3d1-iCre/+; B6-G/R mouse mice, we found that the tdTomato signal was expressed in parietal trophoblast giant cells (P-TGCs). Thus, we report a mouse line with ectopic Cre expression in P-TGCs, which provides a valuable tool for studying human pathological pregnancies caused by implantation failure or abnormal trophoblast secretion due to aberrant gene regulation., (© 2023 Wiley Periodicals LLC.)
- Published
- 2024
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