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Your search keyword '"Daccord, N."' showing total 2 results
Start Over Author "Daccord, N." Topic plant breeding
2 results on '"Daccord, N."'

1. Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breeding.

Author

Thieme M, Lanciano S, Balzergue S, Daccord N, Mirouze M, and Bucher E

Subjects
Arabidopsis genetics, DNA Methylation, DNA, Plant metabolism, Gene Expression Regulation, Plant, Oryza genetics, RNA Polymerase II metabolism, Plant Breeding, RNA Polymerase II antagonists & inhibitors, Retroelements
Abstract

Background: Retrotransposons play a central role in plant evolution and could be a powerful endogenous source of genetic and epigenetic variability for crop breeding. To ensure genome integrity several silencing mechanisms have evolved to repress retrotransposon mobility. Even though retrotransposons fully depend on transcriptional activity of the host RNA polymerase II (Pol II) for their mobility, it was so far unclear whether Pol II is directly involved in repressing their activity., Results: Here we show that plants defective in Pol II activity lose DNA methylation at repeat sequences and produce more extrachromosomal retrotransposon DNA upon stress in Arabidopsis and rice. We demonstrate that combined inhibition of both DNA methylation and Pol II activity leads to a strong stress-dependent mobilization of the heat responsive ONSEN retrotransposon in Arabidopsis seedlings. The progenies of these treated plants contain up to 75 new ONSEN insertions in their genome which are stably inherited over three generations of selfing. Repeated application of heat stress in progeny plants containing increased numbers of ONSEN copies does not result in increased activation of this transposon compared to control lines. Progenies with additional ONSEN copies show a broad panel of environment-dependent phenotypic diversity., Conclusions: We demonstrate that Pol II acts at the root of transposon silencing. This is important because it suggests that Pol II can regulate the speed of plant evolution by fine-tuning the amplitude of transposon mobility. Our findings show that it is now possible to study induced transposon bursts in plants and unlock their use to induce epigenetic and genetic diversity for crop breeding.

Published
2017
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2. A high-quality genome sequence of Rosa chinensis to elucidate ornamental traits

Author

Hibrand Saint-Oyant, L., Ruttink, T., Hamama, L., Kirov, I., Lakhwani, D., Zhou, N. N., Bourke, P. M., Daccord, N., Leus, L., Schulz, D., Van de Geest, H., Hesselink, T., Van Laere, K., Debray, K., Balzergue, S., Thouroude, T., Chastellier, A., Jeauffre, J., Voisine, L., Gaillard, S., Borm, T. J. A., Arens, P., Voorrips, R. E., Maliepaard, C., Neu, E., Linde, M., Le Paslier, M. C., Bérard, A., Bounon, R., Clotault, J., Choisne, N., Quesneville, H., Kawamura, K., Aubourg, S., Sakr, S., Smulders, M. J. M., Schijlen, E., Bucher, E., Debener, T., De Riek, J., Foucher, F., Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institute for Agricultural and Fisheries Research (ILVO), Institute for Agricultural and Fisheries Research, Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazev, Wageningen University and Research Centre [Wageningen] (WUR), Leibniz Universität Hannover [Hannover] (LUH), Etude du Polymorphisme des Génomes Végétaux (EPGV), Institut National de la Recherche Agronomique (INRA), Unité de Recherche Génomique Info (URGI), Osaka Institute of Technology (OIT), ANR-13-BSV7-0014,DODO,Identification et caractérisation des mutations DOMINANT DOUBLE (DODO) and DOUBLE FLOWER (DF) chez le pétunia et la rose(2013), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Research Institute for Agricultural, Fisheries and Food (ILVO), Wageningen University and Research [Wageningen] (WUR), Osaka Institute of Technology = Osaka Kogyo Daigaku (OIT), CEA-IG/CNG, ‘Région Pays de la Loire’ (Rose Genome Project, Genorose project in the framework of RFI ‘Objectif Végétal’, EPICENTER ConnecTalent grant of the Pays de la Loire), ANR [ANR-13-BSV7-0014], JSPS [JSPS KAKENHI no.17H04616], German Ministry of Economic Affairs (Aif programme ZI), Deutsche Forschungsgemeinschaft [DFG program GRK1798], TTI Green Genetics, TKI Polyploids projects [BO-26.03-002-001, BO-50-002-022], AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Leibniz Universität Hannover=Leibniz University Hannover, Cliquet, Catherine, and Blanc 2013 - Identification et caractérisation des mutations DOMINANT DOUBLE (DODO) and DOUBLE FLOWER (DF) chez le pétunia et la rose - - DODO2013 - ANR-13-BSV7-0014 - Blanc 2013 - VALID

Subjects
Male, GENUS ROSA, Arabidopsis, Haploidy, TETRAPLOID ROSE, Laboratorium voor Plantenveredeling, Genome structure, PBR Siergewassen, Tissue Culture, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.SA.HORT]Life Sciences [q-bio]/Agricultural sciences/Horticulture, In Situ Hybridization, Fluorescence, Phylogeny, PBR Kwantitatieve aspecten, food and beverages, PBR Ornamentals, tissue culture and gene transfer, Agriculture, Genomics, PE&RC, READ ALIGNMENT, ddc:580, BIOS Applied Metabolic Systems, PBR Biodiversity and genetic variation, Genome, Plant, Agricultural genetics, GENES, SELF-INCOMPATIBILITY, Centromere, Quantitative Trait Loci, Flowers, Rosa, Fragaria, Synteny, Article, Chromosomes, Plant, PBR Quantitative aspects of Plant Breeding, BIOS Applied Bioinformatics, PBR Biodiversiteit en Genetische Variatie, [SDV.SA.HORT] Life Sciences [q-bio]/Agricultural sciences/Horticulture, Quantitative Trait, Heritable, Genetics, Humans, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Life Science, FLOWER DEVELOPMENT, Konferenzschrift, Genetic association study, Indoleacetic Acids, Arabidopsis Proteins, fungi, Genetic Variation, Sequence Analysis, DNA, MOLECULAR EVOLUTION, DNA-SEQUENCES, Plant Breeding, Fertility, ARABIDOPSIS-THALIANA, TFL1 HOMOLOG, Rosa chinensis, EPS
Abstract

Rose is the world’s most important ornamental plant, with economic, cultural and symbolic value. Roses are cultivated worldwide and sold as garden roses, cut flowers and potted plants. Roses are outbred and can have various ploidy levels. Our objectives were to develop a high-quality reference genome sequence for the genus Rosa by sequencing a doubled haploid, combining long and short reads, and anchoring to a high-density genetic map, and to study the genome structure and genetic basis of major ornamental traits. We produced a doubled haploid rose line (‘HapOB’) from Rosa chinensis ‘Old Blush’ and generated a rose genome assembly anchored to seven pseudo-chromosomes (512 Mb with N50 of 3.4 Mb and 564 contigs). The length of 512 Mb represents 90.1–96.1% of the estimated haploid genome size of rose. Of the assembly, 95% is contained in only 196 contigs. The anchoring was validated using high-density diploid and tetraploid genetic maps. We delineated hallmark chromosomal features, including the pericentromeric regions, through annotation of transposable element families and positioned centromeric repeats using fluorescent in situ hybridization. The rose genome displays extensive synteny with the Fragaria vesca genome, and we delineated only two major rearrangements. Genetic diversity was analysed using resequencing data of seven diploid and one tetraploid Rosa species selected from various sections of the genus. Combining genetic and genomic approaches, we identified potential genetic regulators of key ornamental traits, including prickle density and the number of flower petals. A rose APETALA2/TOE homologue is proposed to be the major regulator of petal number in rose. This reference sequence is an important resource for studying polyploidization, meiosis and developmental processes, as we demonstrated for flower and prickle development. It will also accelerate breeding through the development of molecular markers linked to traits, the identification of the genes underlying them and the exploitation of synteny across Rosaceae., A high-quality reference genome of rose is generated by sequencing a doubled haploid line. The researchers analysed the genome structure and uncovered candidate genes associated with major ornamental traits through integrated genetic and genomic analyses.

Published
2018
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