8 results on '"Ulvskov, Peter"'
Search Results
2. Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases
- Author
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Petersen, Bent Larsen, Egelund, Jack, Damager, Iben, Faber, Kirsten, Krüger Jensen, Jacob, Yang, Zhang, Bennett, Eric Paul, Scheller, Henrik Vibe, and Ulvskov, Peter
- Published
- 2009
- Full Text
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3. Molecular characterization of two Arabidopsis thaliana glycosyltransferase mutants, rra1 and rra2, which have a reduced residual arabinose content in a polymer tightly associated with the cellulosic wall residue
- Author
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Egelund, Jack, Obel, Nicolai, Ulvskov, Peter, Geshi, Naomi, Pauly, Markus, Bacic, Antony, and Petersen, Bent Larsen
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- 2007
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4. Evidence for land plant cell wall biosynthetic mechanisms in charophyte green algae.
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Mikkelsen, Maria D., Harholt, Jesper, Ulvskov, Peter, Johansen, Ida E., Fangel, Jonatan U., Doblin, Monika S., Bacic, Antony, and Willats, William G. T.
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PLANT cell walls ,GREEN algae ,BIOSYNTHESIS ,BIOCHEMICAL engineering ,CHAROPHYTA - Abstract
Background and Aims The charophyte green algae (CGA) are thought to be the closest living relatives to the land plants, and ancestral CGA were unique in giving rise to the land plant lineage. The cell wall has been suggested to be a defining structure that enabled the green algal ancestor to colonize land. These cell walls provide support and protection, are a source of signalling molecules, and provide developmental cues for cell differentiation and elongation. The cell wall of land plants is a highly complex fibre composite, characterized by cellulose cross-linked by non-cellulosic polysaccharides, such as xyloglucan, embedded in a matrix of pectic polysaccharides. How the land plant cell wall evolved is currently unknown: early-divergent chlorophyte and prasinophyte algae genomes contain a low number of glycosyl transferases (GTs), while land plants contain hundreds. The number of GTs in CGA is currently unknown, as no genomes are available, so this study sought to give insight into the evolution of the biosynthetic machinery of CGA through an analysis of available transcriptomes. Methods Available CGA transcriptomes were mined for cell wall biosynthesis GTs and compared with GTs characterized in land plants. In addition, gene cloning was employed in two cases to answer important evolutionary questions. Key Results Genetic evidence was obtained indicating that many of the most important core cell wall polysaccharides have their evolutionary origins in the CGA, including cellulose, mannan, xyloglucan, xylan and pectin, as well as arabino-galactan protein. Moreover, two putative cellulose synthase-like D family genes (CSLDs) from the CGA species Coleochaete orbicularis and a fragment of a putative CSLA/K-like sequence from a CGA Spirogyra species were cloned, providing the first evidence that all the cellulose synthase/-like genes present in early-divergent land plants were already present in CGA. Conclusions The results provide new insights into the evolution of cell walls and support the notion that the CGA were pre-adapted to life on land by virtue of the their cell wall biosynthetic capacity. These findings are highly significant for understanding plant cell wall evolution as they imply that some features of land plant cell walls evolved prior to the transition to land, rather than having evolved as a result of selection pressures inherent in this transition. [ABSTRACT FROM PUBLISHER]
- Published
- 2014
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5. A β-glucuronosyltransferase from Arabidopsis thaliana involved in biosynthesis of type II arabinogalactan has a role in cell elongation during seedling growth.
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Knoch, Eva, Dilokpimol, Adiphol, Tryfona, Theodora, Poulsen, Christian P., Xiong, Guangyan, Harholt, Jesper, Petersen, Bent L., Ulvskov, Peter, Hadi, Masood Z., Kotake, Toshihisa, Tsumuraya, Yoichi, Pauly, Markus, Dupree, Paul, and Geshi, Naomi
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ARABIDOPSIS thaliana ,GLUCURONOSYLTRANSFERASE ,BIOSYNTHESIS ,ARABINOGALACTAN ,SEEDLINGS ,PLANT growth ,PLANT enzymes - Abstract
We have characterized a β-glucuronosyltransferase ( At Glc AT14 A) from Arabidopsis thaliana that is involved in the biosynthesis of type II arabinogalactan ( AG). This enzyme belongs to the Carbohydrate Active Enzyme database glycosyltransferase family 14 ( GT14). The protein was localized to the Golgi apparatus when transiently expressed in Nicotiana benthamiana. The soluble catalytic domain expressed in Pichia pastoris transferred glucuronic acid ( Glc A) to β-1,6-galactooligosaccharides with degrees of polymerization ( DP) ranging from 3-11, and to β-1,3-galactooligosaccharides of DP5 and 7, indicating that the enzyme is a glucuronosyltransferase that modifies both the β-1,6- and β-1,3-galactan present in type II AG. Two allelic T- DNA insertion mutant lines showed 20-35% enhanced cell elongation during seedling growth compared to wild-type. Analyses of AG isolated from the mutants revealed a reduction of Glc A substitution on Gal-β-1,6-Gal and β-1,3-Gal, indicating an in vivo role of At Glc AT14A in synthesis of those structures in type II AG. Moreover, a relative increase in the levels of 3-, 6- and 3,6-linked galactose (Gal) and reduced levels of 3-, 2- and 2,5-linked arabinose (Ara) were seen, suggesting that the mutation in AtGlc AT14A results in a relative increase of the longer and branched β-1,3- and β-1,6-galactans. This increase of galactosylation in the mutants is most likely caused by increased availability of the O6 position of Gal, which is a shared acceptor site for At Glc AT14 A and galactosyltransferases in synthesis of type II AG, and thus addition of Glc A may terminate Gal chain extension. We discuss a role for the glucuronosyltransferase in the biosynthesis of type II AG, with a biological role during seedling growth. [ABSTRACT FROM AUTHOR]
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- 2013
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6. Cell wall evolution and diversity.
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Fangel, Jonatan U., Ulvskov, Peter, Knox, J. P., Mikkelsen, Maria D., Harholt, Jesper, Popper, Zoë A., and Willats, William G. T.
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PLANT cell walls ,PLANT cells & tissues ,PLANT diversity ,POLYSACCHARIDES ,PLANT physiology ,BOTANY - Abstract
Plant cell walls display a considerable degree of diversity in their compositions and molecular architectures. In some cases the functional significance of a particular cell wall type appears to be easy to discern: secondary cells walls are often reinforced with lignin that provides durability; the thin cell walls of pollen tubes have particular compositions that enable their tip growth; lupin seed cell walls are characteristically thickened with galactan used as a storage polysaccharide. However, more frequently the evolutionary mechanisms and selection pressures that underpin cell wall diversity and evolution are unclear. For diverse green plants (chlorophytes and streptophytes) the rapidly increasing availability of transcriptome and genome data sets, the development of methods for cell wall analyses which require less material for analysis, and expansion of molecular probe sets, are providing new insights into the diversity and occurrence of cell wall polysac-charides and associated biosynthetic genes. Such research is important for refining our understanding of some of the fundamental processes that enabled plants to colonize land and to subsequently radiate so comprehensively. The study of cell wall structural diversity is also an important aspect of the industrial utilization of global polysaccharide bio-resources [ABSTRACT FROM AUTHOR]
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- 2012
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7. Hemicelluloses.
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Scheller, Henrik Vibe and Ulvskov, Peter
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XYLANS , *GLUCANS , *HEMICELLULOSE , *PLANT cell walls , *LIGNINS - Abstract
Hemicelluloses are polysaccharides in plant cell walls that have β-(1→4)-linked backbones with an equatorial configuration. Hemicelluloses include xyloglucans, xylans, mannans and glucomannans, and β-(1→3,1→4)-glucans. These types of hemicelluloses are present in the cell walls of all terrestrial plants, except for β-(1→3,1→4)-glucans, which are restricted to Poales and a few other groups. The detailed structure of the hemicelluloses and their abundance vary widely between different species and cell types. The most important biological role of hemicelluloses is their contribution to strengthening the cell wall by interaction with cellulose and, in some walls, with lignin. These features are discussed in relation to widely accepted models of the primary wall. Hemicelluloses are synthesized by glycosyltransferases located in the Golgi membranes. Many glycosyltransferases needed for biosynthesis of xyloglucans and mannans are known. In contrast, the biosynthesis of xylans and β-(1→3,1→4)-glucans remains very elusive, and recent studies have led to more questions than answers. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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8. Analytical implications of different methods for preparing plant cell wall material.
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Fangel, Jonatan U., Jones, Catherine Y., Ulvskov, Peter, Harholt, Jesper, and Willats, William G.T.
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PLANT cell walls , *CELL anatomy , *CELL analysis , *BACTERIAL cell walls , *CARBOHYDRATES - Abstract
• Preparation of an Alcohol Insoluble Residue (AIR) is an important first step in the analysis of cell wall carbohydrates. • Variation in the protocols used for AIR preparation impacts the downstream extractability and detection of cell wall components. • Carbohydrate microarrays were used to analyse carbohydrate extractability. • 70-CHCl3/MeOH L protocol can be considered a reasonable starting point for optimisation of carbohydrate extraction. Almost all plant cells are surrounded by a wall constructed of co-extensive networks of polysaccharides and proteoglycans. The capability to analyse cell wall components is essential for both understanding their complex biology and to fully exploit their numerous practical applications. Several biochemical and immunological techniques are used to analyse cell walls and in almost all cases the first step is the preparation of an alcohol insoluble residue (AIR). There is significant variation in the protocols used for AIR preparation, which can have a notable impact on the downstream extractability and detection of cell wall components. To explore these effects, we have formally compared ten AIR preparation methods and analysed polysaccharides subsequently extracted using high-performance anion exchange chromatography (HPAEC-PAD) and Micro Array Polymer Profiling (MAPP). Our results reveal the impact that AIR preparation has on downstream detection of cell wall components and the need for optimisation and consistency when preparing AIR. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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